Construction and Screening of Libraries of Peptide and Non-Peptide Structures

Michal Lebl, Viktor Krchňák, Pavel Šafář, Alena Stierandová, Nikolai F. Sepetov, Petr Kočiš, Kit Lam

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

This chapter describes the construction and screening of libraries of peptide and nonpeptide structures. A necessary requirement for incorporating building blocks other than amino acids or nucleotides into a library to increase diversity is the ability to identify the composition of positively reacting compounds. This is straightforward for library technologies in which the stepwise tracking of synthesis leads. To achieve reasonable binding to a receptor, antibody, enzyme, and nucleic acid, the appropriate spatial arrangement of the interacting structures must be realized. Linear presentation of building blocks in libraries may not be an optimal format for the selection of the best binding structures. An example of a nonpeptidic scaffold is based on the use of Kemps triacid. In this structure, the three carboxyl groups are constrained to the triaxial conformation.

Original languageEnglish (US)
Pages (from-to)541-548
Number of pages8
JournalTechniques in Protein Chemistry
Volume5
Issue numberC
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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