Construction and Screening of Libraries of Peptide and Non-Peptide Structures

Michal Lebl, Viktor Krchňák, Pavel Šafář, Alena Stierandová, Nikolai F. Sepetov, Petr Kočiš, Kit Lam

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This chapter describes the construction and screening of libraries of peptide and nonpeptide structures. A necessary requirement for incorporating building blocks other than amino acids or nucleotides into a library to increase diversity is the ability to identify the composition of positively reacting compounds. This is straightforward for library technologies in which the stepwise tracking of synthesis leads. To achieve reasonable binding to a receptor, antibody, enzyme, and nucleic acid, the appropriate spatial arrangement of the interacting structures must be realized. Linear presentation of building blocks in libraries may not be an optimal format for the selection of the best binding structures. An example of a nonpeptidic scaffold is based on the use of Kemps triacid. In this structure, the three carboxyl groups are constrained to the triaxial conformation.

Original languageEnglish (US)
Pages (from-to)541-548
Number of pages8
JournalTechniques in Protein Chemistry
Volume5
Issue numberC
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Peptide Library
Libraries
Screening
Scaffolds
Nucleic Acids
Conformations
Nucleotides
Technology
Amino Acids
Antibodies
Enzymes
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Construction and Screening of Libraries of Peptide and Non-Peptide Structures. / Lebl, Michal; Krchňák, Viktor; Šafář, Pavel; Stierandová, Alena; Sepetov, Nikolai F.; Kočiš, Petr; Lam, Kit.

In: Techniques in Protein Chemistry, Vol. 5, No. C, 01.01.1994, p. 541-548.

Research output: Contribution to journalArticle

Lebl, M, Krchňák, V, Šafář, P, Stierandová, A, Sepetov, NF, Kočiš, P & Lam, K 1994, 'Construction and Screening of Libraries of Peptide and Non-Peptide Structures', Techniques in Protein Chemistry, vol. 5, no. C, pp. 541-548. https://doi.org/10.1016/B978-0-12-194710-1.50066-7
Lebl M, Krchňák V, Šafář P, Stierandová A, Sepetov NF, Kočiš P et al. Construction and Screening of Libraries of Peptide and Non-Peptide Structures. Techniques in Protein Chemistry. 1994 Jan 1;5(C):541-548. https://doi.org/10.1016/B978-0-12-194710-1.50066-7
Lebl, Michal ; Krchňák, Viktor ; Šafář, Pavel ; Stierandová, Alena ; Sepetov, Nikolai F. ; Kočiš, Petr ; Lam, Kit. / Construction and Screening of Libraries of Peptide and Non-Peptide Structures. In: Techniques in Protein Chemistry. 1994 ; Vol. 5, No. C. pp. 541-548.
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