Construction and in vitro characterization of attenuated feline immunodeficiency virus long terminal repeat mutant viruses

L. Bigornia, K. M. Lockridge, E. E. Sparger

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

AP-1- and ATF-binding sites are cis-acting transcriptional elements within the U3 domain of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) that serve as targets for cellular activation pathways and may regulate virus replication. We report that FIV LTR mutant proviruses encoding U3 deletions of the ATF-binding sequence exhibited restricted virus expression and replication in both feline lymphocytes and macrophages. In contrast, deletion of the AP-1 site had negligible effects on virus expression and replication. FIV LTR mutant proviruses encoding deletions of both the AP-1 and ATF sites or a 72-bp deletion encompassing the AP-1 site, duplicated C/EBP sites, and ATF sites were severely restricted for virus expression. These results demonstrate that deletion of either the ATF-binding site or multiple cis-acting transcriptional elements attenuates FIV. These attenuated FIV mutants provide opportunities to characterize the role of cis-acting elements in virus replication in vivo and to test LTR mutants as attenuated virus vaccines.

Original languageEnglish (US)
Pages (from-to)1054-1060
Number of pages7
JournalJournal of Virology
Volume75
Issue number2
DOIs
StatePublished - 2001

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Feline Immunodeficiency Virus
Feline immunodeficiency virus
terminal repeat sequences
Terminal Repeat Sequences
Transcription Factor AP-1
Virus Replication
Viruses
viruses
mutants
proviruses
Proviruses
virus replication
binding sites
Binding Sites
Attenuated Vaccines
Felidae
macrophages
lymphocytes
Macrophages
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Construction and in vitro characterization of attenuated feline immunodeficiency virus long terminal repeat mutant viruses. / Bigornia, L.; Lockridge, K. M.; Sparger, E. E.

In: Journal of Virology, Vol. 75, No. 2, 2001, p. 1054-1060.

Research output: Contribution to journalArticle

Bigornia, L. ; Lockridge, K. M. ; Sparger, E. E. / Construction and in vitro characterization of attenuated feline immunodeficiency virus long terminal repeat mutant viruses. In: Journal of Virology. 2001 ; Vol. 75, No. 2. pp. 1054-1060.
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