Construction and characterization of two infectious molecular clones of encephalomyocarditis virus

Robert K. Naviaux, Stuart H Cohen, Kurt M. Vanden Brink, George W. Jordan

Research output: Contribution to journalArticle

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Abstract

We constructed and characterized two infectious molecular clones of encephalomyocarditis (EMC) virus. Both constructs, pDL and pDA, were assembled from five overlapping cDNA clones derived from the diabetogenic variant of EMC virus (EMC-D) and from two synthetic oligonucleotide cartridges. pDA contained a single point mutation at position 1720 within the "puff" region of capsid protein 1AB that was derived from the nondiabetogenic variant of EMC virus (EMC-B). This point mutation resulted in an amino acid substitution of arginine (EMC-B) for lysine (EMC-D). Our construction illustrates two novel findings: (i) that the problem of stably cloning long poly(C) tracts of EMC virus can be circumvented by the use of a shortened, synthetic, poly(dC-dG) oligonucleotide cartridge, and (ii) that a single point mutation in the puff region of the capsid protein 1AB leads to change in its electrophoretic mobility and to a change in the plaque size of recombinant virus.

Original languageEnglish (US)
Pages (from-to)913-917
Number of pages5
JournalJournal of Virology
Volume64
Issue number2
StatePublished - Feb 1990

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Encephalomyocarditis virus
point mutation
Clone Cells
Point Mutation
clones
Capsid Proteins
oligonucleotides
coat proteins
Oligonucleotides
Poly C
amino acid substitution
Amino Acid Substitution
Lysine
arginine
Arginine
Organism Cloning
molecular cloning
lysine
Complementary DNA
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Construction and characterization of two infectious molecular clones of encephalomyocarditis virus. / Naviaux, Robert K.; Cohen, Stuart H; Vanden Brink, Kurt M.; Jordan, George W.

In: Journal of Virology, Vol. 64, No. 2, 02.1990, p. 913-917.

Research output: Contribution to journalArticle

Naviaux, Robert K. ; Cohen, Stuart H ; Vanden Brink, Kurt M. ; Jordan, George W. / Construction and characterization of two infectious molecular clones of encephalomyocarditis virus. In: Journal of Virology. 1990 ; Vol. 64, No. 2. pp. 913-917.
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