Constrained tibial vibration in mice: A method for studying the effects of vibrational loading of bone

Blaine A Christiansen, Philip V. Bayly, Matthew J. Silva

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Vibrational loading can stimulate the formation of new trabecular bone or maintain bone mass. Studies investigating vibrational loading have often used whole-body vibration (WBV) as their loading method. However, WBV has limitations in small animal studies because transmissibility of vibration is dependent on posture. In this study, we propose constrained tibial vibration (CTV) as an experimental method for vibrational loading of mice under controlled conditions. In CTV, the lower leg of an anesthetized mouse is subjected to vertical vibrational loading while supporting a mass. The setup approximates a one degree-of-freedom vibrational system. Accelerometers were used to measure transmissibility of vibration through the lower leg in CTV at frequencies from 20 Hz to 150 Hz. First, the frequency response of transmissibility was quantified in vivo, and dissections were performed to remove one component of the mouse leg (the knee joint, foot, or soft tissue) to investigate the contribution of each component to the frequency response of the intact leg. Next, a finite element (FE) model of a mouse tibia-fibula was used to estimate the deformation of the bone during CTV. Finally, strain gages were used to determine the dependence of bone strain on loading frequency. The in vivo mouse leg in the CTV system had a resonant frequency of 60 Hz for ±0.5 G vibration (1.0 G peak to peak). Removing the foot caused the natural frequency of the system to shift from 60 Hz to 70 Hz, removing the soft tissue caused no change in natural frequency, and removing the knee changed the natural frequency from 60 Hz to 90 Hz. By using the FE model, maximum tensile and compressive strains during CTV were estimated to be on the cranial-medial and caudolateral surfaces of the tibia, respectively, and the peak transmissibility and peak cortical strain occurred at the same frequency. Strain gage data confirmed the relationship between peak transmissibility and peak bone strain indicated by the FE model, and showed that the maximum cyclic tibial strain during CTV of the intact leg was 330±82με and occurred at 60-70 Hz. This study presents a comprehensive mechanical analysis of CTV a loading method for studying vibrational loading under controlled conditions. This model will be used in future in vivo studies and will potentially become an important tool for understanding the response of bone to vibrational loading.

Original languageEnglish (US)
Article number044502
JournalJournal of Biomechanical Engineering
Volume130
Issue number4
DOIs
StatePublished - Aug 2008
Externally publishedYes

Fingerprint

Vibration
Bone
Bone and Bones
Natural frequencies
Leg
Strain gages
Frequency response
Tissue
Dissection
Tibia
Foot
Accelerometers
Vibrations (mechanical)
Animals
Fibula
Knee Joint
Posture
Knee

Keywords

  • Osteoporosis
  • Transmissibility
  • Vibration
  • WBV

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biophysics
  • Physiology (medical)

Cite this

Constrained tibial vibration in mice : A method for studying the effects of vibrational loading of bone. / Christiansen, Blaine A; Bayly, Philip V.; Silva, Matthew J.

In: Journal of Biomechanical Engineering, Vol. 130, No. 4, 044502, 08.2008.

Research output: Contribution to journalArticle

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