Considerations for potency equivalent calculations in the Ah receptor-based CALUX bioassay: Normalization of superinduction results for improved sample potency estimation

David S. Baston, Michael S. Denison

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The chemically activated luciferase expression (CALUX) system is a mechanistically based recombinant luciferase reporter gene cell bioassay used in combination with chemical extraction and clean-up methods for the detection and relative quantitation of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like halogenated aromatic hydrocarbons in a wide variety of sample matrices. While sample extracts containing complex mixtures of chemicals can produce a variety of distinct concentration-dependent luciferase induction responses in CALUX cells, these effects are produced through a common mechanism of action (i.e. the Ah receptor (AhR)) allowing normalization of results and sample potency determination. Here we describe the diversity in CALUX response to PCDD/Fs from sediment and soil extracts and not only report the occurrence of superinduction of the CALUX bioassay, but we describe a mechanistically based approach for normalization of superinduction data that results in a more accurate estimation of the relative potency of such sample extracts.

Original languageEnglish (US)
Pages (from-to)1415-1421
Number of pages7
JournalTalanta
Volume83
Issue number5
DOIs
StatePublished - Feb 15 2011

Keywords

  • 2-3-7-8-Tetrachlorodibenzo-p-dioxin
  • Ah receptor
  • Bioassay
  • CALUX
  • Superinduction
  • TCDD

ASJC Scopus subject areas

  • Chemistry(all)

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