TY - JOUR
T1 - Conserving a volatile metabolite
T2 - A role for carboxysome-like organelles in Salmonella enterica
AU - Penrod, Joseph T.
AU - Roth, John R.
PY - 2006/4
Y1 - 2006/4
N2 - Salmonellae can use ethanolamine (EA) as a sole source of carbon and nitrogen. This ability is encoded by an operon (eut) containing 17 genes, only 6 of which are required under standard conditions (37°C; pH 7.0). Five of the extra genes (eutM, -N, -L, -K, and -G) become necessary under conditions that favor loss of the volatile intermediate, acetaldehyde, which escapes as a gas during growth on EA and is lost at a higher rate from these mutants. The eutM, -N, -L, and -K genes encode homologues of shell proteins of the carboxysome, an organelle shown (in other organisms) to concentrate CO2. We propose that carboxysome-like organelles help bacteria conserve certain volatile metabolites-CO2 or acetaldehyde-perhaps by providing a low-pH compartment. The EutG enzyme converts acetaldehyde to ethanol, which may improve carbon retention by forming acetals; alternatively, EutG may recycle NADH within the carboxysome.
AB - Salmonellae can use ethanolamine (EA) as a sole source of carbon and nitrogen. This ability is encoded by an operon (eut) containing 17 genes, only 6 of which are required under standard conditions (37°C; pH 7.0). Five of the extra genes (eutM, -N, -L, -K, and -G) become necessary under conditions that favor loss of the volatile intermediate, acetaldehyde, which escapes as a gas during growth on EA and is lost at a higher rate from these mutants. The eutM, -N, -L, and -K genes encode homologues of shell proteins of the carboxysome, an organelle shown (in other organisms) to concentrate CO2. We propose that carboxysome-like organelles help bacteria conserve certain volatile metabolites-CO2 or acetaldehyde-perhaps by providing a low-pH compartment. The EutG enzyme converts acetaldehyde to ethanol, which may improve carbon retention by forming acetals; alternatively, EutG may recycle NADH within the carboxysome.
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U2 - 10.1128/JB.188.8.2865-2874.2006
DO - 10.1128/JB.188.8.2865-2874.2006
M3 - Article
C2 - 16585748
AN - SCOPUS:33645964606
VL - 188
SP - 2865
EP - 2874
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 8
ER -