Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes

Robert B. Hufnagel, Amy N. Riesenberg, Sara M. Saul, Nadean L Brown

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Retinal ganglion cell genesis requires the proneural bHLH transcription factor Math5 (Atoh7), but little is known about the regulatory elements that control its expression. Here, we investigate Math5 gene regulation using transgenic mice. These mice express GFP in the prenatal retina, live-labeling RGC axon migration and innervation of the brain. Unexpectedly, these Math5-GFP transgenes are also found in Math1 expression domains throughout the nervous system, intriguing since Math5 and Math1 normally exhibit nonoverlapping expression. Furthermore, Math5-GFP and Math1 are regulated similarly, by both Pax6 and Math1 itself, in the lower rhombic lip and dorsal spinal cord. We also show that Pax6 binds to particular Math5 and Math1 regulatory sequences in vitro. Together these data suggest that these atonal semi-orthologues may share conserved regulatory elements that are normally silent in the Math5 gene.

Original languageEnglish (US)
Pages (from-to)435-448
Number of pages14
JournalMolecular and Cellular Neuroscience
Issue number4
StatePublished - Dec 2007
Externally publishedYes


  • Cochlear nucleus
  • Math1
  • Math5
  • Optic nerve
  • Pax6
  • Visual System

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience


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