Conserved organization of the murine immunoglobulin ε gene region: Restriction endonuclease maps and switch-region nucleotide sequence

C. A. Gritzmacher, Fu-Tong Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Various inbred mouse strains showed remarkable conservation of organization in the ε germline region as determined by restriction mapping. Restriction fragment length polymorphisms (RFLP) were seen in the ε gene region for only two of eight restriction enzymes tested. Furthermore, the RFLP did not correlate with the IgE-response phenotype for the murine strains SJL, SJA, C57BL/6, BALB/c, A/ST, and A/J. The IgE class-switch region (Sε) DNA from an SJL genomic clone was sequenced and was compared with Sε sequences from BALB/c mice. These Sε sequences were at least 95% homologous. Most of the Sε sequence differences observed between the two strains were single base pair substitutions, deletions, or insertions. The largest difference between the Sε sequences resulted from an insertion of seven contiguous bases seen in the SJL Sε region.

Original languageEnglish (US)
Pages (from-to)603-607
Number of pages5
JournalJournal of Immunology
Volume139
Issue number2
StatePublished - 1987

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Immunoglobulin Genes
DNA Restriction Enzymes
Restriction Fragment Length Polymorphisms
Immunoglobulin E
Restriction Mapping
Inbred Strains Mice
Insertional Mutagenesis
Base Pairing
Clone Cells
Phenotype
DNA
Enzymes
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Conserved organization of the murine immunoglobulin ε gene region : Restriction endonuclease maps and switch-region nucleotide sequence. / Gritzmacher, C. A.; Liu, Fu-Tong.

In: Journal of Immunology, Vol. 139, No. 2, 1987, p. 603-607.

Research output: Contribution to journalArticle

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