Abstract
Although drug-induced and age-related hearing losses are frequent otologic problems affecting millions of people, their underlying mechanisms remain uncertain. The inner ear is exclusively endowed with a positive endocochlear potential (EP) that serves as the main driving force for the generation of receptor potential in hair cells to confer hearing. Deterioration of EP leads to hearing loss or deafness. The generation of EP relies on the activity of many ion transporters to establish active potassium (K+) cycling within the inner ear, including K+ channels, the Na-K-2Cl co-transporter (NKCC1), and the α1 and α2 isoforms of Na,K-ATPase. We show that heterozygous deletion of either NKCC1, α1-Na,K-ATPase, or α2-Na,K-ATPase independently results in progressive, age-dependent hearing loss with minimal alteration in cochlear morphology. Double heterozygote deletion of NKCC1 with α1-Na,K-ATPase also shows a progressive, though delayed, age-dependent hearing loss. Remarkably, double heterozygote deletion of NKCC1 with α2-Na,K-ATPase demonstrates a striking preservation of hearing threshold both initially and with age. Measurements of the EP confirm the anticipated drop in potential for genotypes that demonstrate age-dependent hearing loss. The EP generated by the NKCC1 + α2-Na,K-ATPase double heterozygote, however, is maintained at a level comparable to that of the control condition, suggesting a potential advantage in this combination of ion transporter modification. These observations provide insight into the detailed mechanisms of EP generation, and results of combination-knockout experiments may have important implications in the future treatment of drug-induced and age-related hearing losses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 422-434 |
| Number of pages | 13 |
| Journal | JARO - Journal of the Association for Research in Otolaryngology |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2007 |
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Keywords
- Age-related hearing loss
- Endocochlear potential
- Gene targeting
- Potassium transport
- Stria vascularis
ASJC Scopus subject areas
- Otorhinolaryngology
- Physiology
Cite this
Conservation of hearing by simultaneous mutation of Na,K-ATPase and NKCC1. / Diaz, Rodney C; Vazquez, Ana Elena; Dou, Hongwei; Wei, Dongguang; Cardell, Emma Lou; Lingrel, Jerry; Shull, Gary E.; Doyle, Karen Jo; Yamoah, Ebenezer N.
In: JARO - Journal of the Association for Research in Otolaryngology, Vol. 8, No. 4, 12.2007, p. 422-434.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Conservation of hearing by simultaneous mutation of Na,K-ATPase and NKCC1
AU - Diaz, Rodney C
AU - Vazquez, Ana Elena
AU - Dou, Hongwei
AU - Wei, Dongguang
AU - Cardell, Emma Lou
AU - Lingrel, Jerry
AU - Shull, Gary E.
AU - Doyle, Karen Jo
AU - Yamoah, Ebenezer N.
PY - 2007/12
Y1 - 2007/12
N2 - Although drug-induced and age-related hearing losses are frequent otologic problems affecting millions of people, their underlying mechanisms remain uncertain. The inner ear is exclusively endowed with a positive endocochlear potential (EP) that serves as the main driving force for the generation of receptor potential in hair cells to confer hearing. Deterioration of EP leads to hearing loss or deafness. The generation of EP relies on the activity of many ion transporters to establish active potassium (K+) cycling within the inner ear, including K+ channels, the Na-K-2Cl co-transporter (NKCC1), and the α1 and α2 isoforms of Na,K-ATPase. We show that heterozygous deletion of either NKCC1, α1-Na,K-ATPase, or α2-Na,K-ATPase independently results in progressive, age-dependent hearing loss with minimal alteration in cochlear morphology. Double heterozygote deletion of NKCC1 with α1-Na,K-ATPase also shows a progressive, though delayed, age-dependent hearing loss. Remarkably, double heterozygote deletion of NKCC1 with α2-Na,K-ATPase demonstrates a striking preservation of hearing threshold both initially and with age. Measurements of the EP confirm the anticipated drop in potential for genotypes that demonstrate age-dependent hearing loss. The EP generated by the NKCC1 + α2-Na,K-ATPase double heterozygote, however, is maintained at a level comparable to that of the control condition, suggesting a potential advantage in this combination of ion transporter modification. These observations provide insight into the detailed mechanisms of EP generation, and results of combination-knockout experiments may have important implications in the future treatment of drug-induced and age-related hearing losses.
AB - Although drug-induced and age-related hearing losses are frequent otologic problems affecting millions of people, their underlying mechanisms remain uncertain. The inner ear is exclusively endowed with a positive endocochlear potential (EP) that serves as the main driving force for the generation of receptor potential in hair cells to confer hearing. Deterioration of EP leads to hearing loss or deafness. The generation of EP relies on the activity of many ion transporters to establish active potassium (K+) cycling within the inner ear, including K+ channels, the Na-K-2Cl co-transporter (NKCC1), and the α1 and α2 isoforms of Na,K-ATPase. We show that heterozygous deletion of either NKCC1, α1-Na,K-ATPase, or α2-Na,K-ATPase independently results in progressive, age-dependent hearing loss with minimal alteration in cochlear morphology. Double heterozygote deletion of NKCC1 with α1-Na,K-ATPase also shows a progressive, though delayed, age-dependent hearing loss. Remarkably, double heterozygote deletion of NKCC1 with α2-Na,K-ATPase demonstrates a striking preservation of hearing threshold both initially and with age. Measurements of the EP confirm the anticipated drop in potential for genotypes that demonstrate age-dependent hearing loss. The EP generated by the NKCC1 + α2-Na,K-ATPase double heterozygote, however, is maintained at a level comparable to that of the control condition, suggesting a potential advantage in this combination of ion transporter modification. These observations provide insight into the detailed mechanisms of EP generation, and results of combination-knockout experiments may have important implications in the future treatment of drug-induced and age-related hearing losses.
KW - Age-related hearing loss
KW - Endocochlear potential
KW - Gene targeting
KW - Potassium transport
KW - Stria vascularis
UR - http://www.scopus.com/inward/record.url?scp=35848962970&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35848962970&partnerID=8YFLogxK
U2 - 10.1007/s10162-007-0089-4
DO - 10.1007/s10162-007-0089-4
M3 - Article
C2 - 17674100
AN - SCOPUS:35848962970
VL - 8
SP - 422
EP - 434
JO - JARO - Journal of the Association for Research in Otolaryngology
JF - JARO - Journal of the Association for Research in Otolaryngology
SN - 1525-3961
IS - 4
ER -