Conservation genomics of desert dwelling California voles (Microtus californicus) and implications for management of endangered Amargosa voles (Microtus californicus scirpensis)

Alexander R. Krohn, Chris J. Conroy, Risa Pesapane, Ke Bi, Janet E Foley, Erica Bree Rosenblum

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Understanding population genetic structure and levels of genetic variation is critical for the conservation and management of imperiled populations, especially when reintroductions are planned. We used restriction-site associated DNA (RAD) sequencing to study the genetic diversity and evolutionary relationships of the endangered Amargosa vole and other closely related desert-dwelling California voles. Specifically, we sought to determine how Amargosa voles are related to other California voles, how genetic variation is partitioned among subpopulations in wild Amargosa voles, and how much genetic variation is captured within a captive insurance colony of Amargosa voles. Our multilocus nuclear dataset provides strong evidence that Amargosa voles are part of a northern clade of California voles. Amargosa voles have highly reduced genetic variation relative to other California voles, but do exhibit some sub-structure among sampled marshes. Captive Amargosa voles capture approximately half of the total genetic variation present in the wild Amargosa vole populations. We discuss the management implications of our findings in light of reintroductions planned for Amargosa voles. Our study highlights the utility of reduced representation genomic approaches, like RADseq, to resolve relationships among small populations that are difficult to study with traditional markers due to low genetic variation and few individuals left in the wild.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalConservation Genetics
DOIs
StateAccepted/In press - Aug 21 2017

Fingerprint

Arvicolinae
Genomics
genetic variation
deserts
genomics
desert
reintroduction
subpopulation
genetic structure
population genetics
dwelling
Microtus californicus
voles
marsh
insurance
DNA
Population
marshes
Wetlands
Genetic Structures

Keywords

  • Captive breeding programs
  • Endangered species
  • Population structure
  • RADseq

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Conservation genomics of desert dwelling California voles (Microtus californicus) and implications for management of endangered Amargosa voles (Microtus californicus scirpensis). / Krohn, Alexander R.; Conroy, Chris J.; Pesapane, Risa; Bi, Ke; Foley, Janet E; Rosenblum, Erica Bree.

In: Conservation Genetics, 21.08.2017, p. 1-13.

Research output: Contribution to journalArticle

@article{c90ac4db2dfd4d588f5177e75211bdad,
title = "Conservation genomics of desert dwelling California voles (Microtus californicus) and implications for management of endangered Amargosa voles (Microtus californicus scirpensis)",
abstract = "Understanding population genetic structure and levels of genetic variation is critical for the conservation and management of imperiled populations, especially when reintroductions are planned. We used restriction-site associated DNA (RAD) sequencing to study the genetic diversity and evolutionary relationships of the endangered Amargosa vole and other closely related desert-dwelling California voles. Specifically, we sought to determine how Amargosa voles are related to other California voles, how genetic variation is partitioned among subpopulations in wild Amargosa voles, and how much genetic variation is captured within a captive insurance colony of Amargosa voles. Our multilocus nuclear dataset provides strong evidence that Amargosa voles are part of a northern clade of California voles. Amargosa voles have highly reduced genetic variation relative to other California voles, but do exhibit some sub-structure among sampled marshes. Captive Amargosa voles capture approximately half of the total genetic variation present in the wild Amargosa vole populations. We discuss the management implications of our findings in light of reintroductions planned for Amargosa voles. Our study highlights the utility of reduced representation genomic approaches, like RADseq, to resolve relationships among small populations that are difficult to study with traditional markers due to low genetic variation and few individuals left in the wild.",
keywords = "Captive breeding programs, Endangered species, Population structure, RADseq",
author = "Krohn, {Alexander R.} and Conroy, {Chris J.} and Risa Pesapane and Ke Bi and Foley, {Janet E} and Rosenblum, {Erica Bree}",
year = "2017",
month = "8",
day = "21",
doi = "10.1007/s10592-017-1010-2",
language = "English (US)",
pages = "1--13",
journal = "Conservation Genetics",
issn = "1566-0621",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Conservation genomics of desert dwelling California voles (Microtus californicus) and implications for management of endangered Amargosa voles (Microtus californicus scirpensis)

AU - Krohn, Alexander R.

AU - Conroy, Chris J.

AU - Pesapane, Risa

AU - Bi, Ke

AU - Foley, Janet E

AU - Rosenblum, Erica Bree

PY - 2017/8/21

Y1 - 2017/8/21

N2 - Understanding population genetic structure and levels of genetic variation is critical for the conservation and management of imperiled populations, especially when reintroductions are planned. We used restriction-site associated DNA (RAD) sequencing to study the genetic diversity and evolutionary relationships of the endangered Amargosa vole and other closely related desert-dwelling California voles. Specifically, we sought to determine how Amargosa voles are related to other California voles, how genetic variation is partitioned among subpopulations in wild Amargosa voles, and how much genetic variation is captured within a captive insurance colony of Amargosa voles. Our multilocus nuclear dataset provides strong evidence that Amargosa voles are part of a northern clade of California voles. Amargosa voles have highly reduced genetic variation relative to other California voles, but do exhibit some sub-structure among sampled marshes. Captive Amargosa voles capture approximately half of the total genetic variation present in the wild Amargosa vole populations. We discuss the management implications of our findings in light of reintroductions planned for Amargosa voles. Our study highlights the utility of reduced representation genomic approaches, like RADseq, to resolve relationships among small populations that are difficult to study with traditional markers due to low genetic variation and few individuals left in the wild.

AB - Understanding population genetic structure and levels of genetic variation is critical for the conservation and management of imperiled populations, especially when reintroductions are planned. We used restriction-site associated DNA (RAD) sequencing to study the genetic diversity and evolutionary relationships of the endangered Amargosa vole and other closely related desert-dwelling California voles. Specifically, we sought to determine how Amargosa voles are related to other California voles, how genetic variation is partitioned among subpopulations in wild Amargosa voles, and how much genetic variation is captured within a captive insurance colony of Amargosa voles. Our multilocus nuclear dataset provides strong evidence that Amargosa voles are part of a northern clade of California voles. Amargosa voles have highly reduced genetic variation relative to other California voles, but do exhibit some sub-structure among sampled marshes. Captive Amargosa voles capture approximately half of the total genetic variation present in the wild Amargosa vole populations. We discuss the management implications of our findings in light of reintroductions planned for Amargosa voles. Our study highlights the utility of reduced representation genomic approaches, like RADseq, to resolve relationships among small populations that are difficult to study with traditional markers due to low genetic variation and few individuals left in the wild.

KW - Captive breeding programs

KW - Endangered species

KW - Population structure

KW - RADseq

UR - http://www.scopus.com/inward/record.url?scp=85027991114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027991114&partnerID=8YFLogxK

U2 - 10.1007/s10592-017-1010-2

DO - 10.1007/s10592-017-1010-2

M3 - Article

AN - SCOPUS:85027991114

SP - 1

EP - 13

JO - Conservation Genetics

JF - Conservation Genetics

SN - 1566-0621

ER -