Abstract
Preparation of the chelating agent (S)-4-[2,3-bis[bis(carboxymethyl)amino]propyl]phenyl isothiocyanate is reported. Procedures for conjugation of this and (S)-N-4-[2,3-bis[bis(carboxymethyl)amino]propyl]phenyl bromoacetamide to monoclonal antibodies and other proteins are described. The conjugates may be purified quickly by centrifugation through Sephadex G-50. The number of protein-bound chelating groups may be measured by titration with standard 57Co2+, using thin-layer chromatography to monitor binding. The labeled products retain their immunoreactivity, as illustrated by experiments in vivo with chelate-conjugated antibody to mouse I-AK antigen.
Original language | English (US) |
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Pages (from-to) | 68-78 |
Number of pages | 11 |
Journal | Analytical Biochemistry |
Volume | 142 |
Issue number | 1 |
DOIs | |
State | Published - 1984 |
Keywords
- gel filtration
- immunoglobulins
- luminescence
- metal ions
- organic synthesis
- radioactive
- radioactivity measurement
- thin-layer chromatography
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology