Conjugation, meiosis, and the osmotic stress response are regulated by Spc1 kinase through Atf1 transcription factor in fission yeast

Kazuhiro Shiozaki, Paul Russell

Research output: Contribution to journalArticle

336 Citations (Scopus)

Abstract

The stress-activated Wis1-Spc1 protein kinase cascade links mitotic control with environmental signals in Schizosaccharomyces pombe. Fission yeast spc1- mutants are delayed in G2 during normal growth and undergo G2 arrest when exposed to osmotic or oxidative stress. Here we report that Spc1 also has an important role in regulating sexual development in S. pombe. This discovery arose from the observation that Spc1 is activated in response to nitrogen limitation, a key signal that promotes conjugation in fission yeast. Mutant spc1- cells are defective at arresting in G1 during nitrogen starvation and exhibit a poor mating ability. These deficiencies correlate with a failure to induce transcription of ste11+, a gene that encodes a transcription factor responsible for expression of various meiotic genes. Two genes, atf1+ and atf21+, were cloned as multicopy suppressors of the spc1 mating defect. Atf1 and Atf21 are bZIP transcription factors that are most closely related to human ATF-2/CRE-BP1. Spc1 is required for stress-induced phosphorylation of Atf1. Atf1 is required for induction of meiotic genes and stress-response genes, such as gpd1+ and pyp2+, that are transcriptionally regulated by Spc1. atf1- and spc1- mutants are sensitive to osmotic stress and impaired for sexual development, showing that fission yeast uses a common pathway to respond to cytotoxic stress and nitrogen starvation. However, unlike spc1- mutants, atf1- cells have no mitotic cell-cycle defect, indicating that the stress response pathway bifurcates at Spc1 to regulate independently meiosis and mitosis.

Original languageEnglish (US)
Pages (from-to)2276-2288
Number of pages13
JournalGenes and Development
Volume10
Issue number18
StatePublished - 1996
Externally publishedYes

Fingerprint

Osmoregulation
Schizosaccharomyces
Meiosis
Transcription Factors
Phosphotransferases
Sexual Development
Nitrogen
Genes
Osmotic Pressure
Starvation
Basic-Leucine Zipper Transcription Factors
Mitosis
Protein Kinases
Cell Cycle
Oxidative Stress
Phosphorylation
Growth

Keywords

  • ATF-2
  • meiosis
  • Schizosaccharomyces pombe
  • stress-activated MAP kinase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Conjugation, meiosis, and the osmotic stress response are regulated by Spc1 kinase through Atf1 transcription factor in fission yeast. / Shiozaki, Kazuhiro; Russell, Paul.

In: Genes and Development, Vol. 10, No. 18, 1996, p. 2276-2288.

Research output: Contribution to journalArticle

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