Congenital transmission of toxoplasma gondii in deer mice (peromyscus maniculatus) after oral oocyst infection

Daniel Rejmanek, Elizabeth Vanwormer, Jonna A Mazet, Andrea E. Packham, Beatriz Aguilar, Patricia A Conrad

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To investigate how different routes of Toxoplasma gondii transmission influence the antibody response and infection status of deer mice (Peromyscus maniculatus), 80 mice were orally infected with 1, 5, 10, or 100 T. gondii oocysts. Ten weeks postinfection, 15 T. gondiiseropositive female mice were bred and allowed to produce 2 litters. Evidence of persistent T .gondii infection in orally infected mice was detected by serology and DNA amplification in mice from all 4 oocyst treatment groups, including those that received only a single T. gondii oocyst. Congenital transmission of T. gondii was detected by polymerase chain reaction (PCR) in 7/8 first and 4/7 second litters. Toxoplasma gondii was also detected by PCR in 9/30 congenitally infected offspring 16 wk after birth, despite the fact that detectable serological titers had waned. These findings raise questions about the applicability of serological testing to assess the prevalence of T. gondii infection in deer mice and other rodents in the wild. Additionally, the detection of frequent congenital transmission suggests that deer mice could help maintain T. gondii in the environment even in the absence of definitive feline hosts.

Original languageEnglish (US)
Pages (from-to)516-520
Number of pages5
JournalJournal of Parasitology
Volume96
Issue number3
DOIs
StatePublished - Jun 2010

Fingerprint

Peromyscus
Peromyscus maniculatus
Oocysts
Toxoplasma
Toxoplasma gondii
oocysts
deer
mouth
polymerase chain reaction
litter
Infection
infection
definitive host
mice
rodent
antibody
amplification
DNA
Polymerase Chain Reaction
Toxoplasmosis

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Congenital transmission of toxoplasma gondii in deer mice (peromyscus maniculatus) after oral oocyst infection. / Rejmanek, Daniel; Vanwormer, Elizabeth; Mazet, Jonna A; Packham, Andrea E.; Aguilar, Beatriz; Conrad, Patricia A.

In: Journal of Parasitology, Vol. 96, No. 3, 06.2010, p. 516-520.

Research output: Contribution to journalArticle

Rejmanek, Daniel ; Vanwormer, Elizabeth ; Mazet, Jonna A ; Packham, Andrea E. ; Aguilar, Beatriz ; Conrad, Patricia A. / Congenital transmission of toxoplasma gondii in deer mice (peromyscus maniculatus) after oral oocyst infection. In: Journal of Parasitology. 2010 ; Vol. 96, No. 3. pp. 516-520.
@article{066815c2d8ff4dfd8672bc83c67fdc98,
title = "Congenital transmission of toxoplasma gondii in deer mice (peromyscus maniculatus) after oral oocyst infection",
abstract = "To investigate how different routes of Toxoplasma gondii transmission influence the antibody response and infection status of deer mice (Peromyscus maniculatus), 80 mice were orally infected with 1, 5, 10, or 100 T. gondii oocysts. Ten weeks postinfection, 15 T. gondiiseropositive female mice were bred and allowed to produce 2 litters. Evidence of persistent T .gondii infection in orally infected mice was detected by serology and DNA amplification in mice from all 4 oocyst treatment groups, including those that received only a single T. gondii oocyst. Congenital transmission of T. gondii was detected by polymerase chain reaction (PCR) in 7/8 first and 4/7 second litters. Toxoplasma gondii was also detected by PCR in 9/30 congenitally infected offspring 16 wk after birth, despite the fact that detectable serological titers had waned. These findings raise questions about the applicability of serological testing to assess the prevalence of T. gondii infection in deer mice and other rodents in the wild. Additionally, the detection of frequent congenital transmission suggests that deer mice could help maintain T. gondii in the environment even in the absence of definitive feline hosts.",
author = "Daniel Rejmanek and Elizabeth Vanwormer and Mazet, {Jonna A} and Packham, {Andrea E.} and Beatriz Aguilar and Conrad, {Patricia A}",
year = "2010",
month = "6",
doi = "10.1645/GE-2372.1",
language = "English (US)",
volume = "96",
pages = "516--520",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "3",

}

TY - JOUR

T1 - Congenital transmission of toxoplasma gondii in deer mice (peromyscus maniculatus) after oral oocyst infection

AU - Rejmanek, Daniel

AU - Vanwormer, Elizabeth

AU - Mazet, Jonna A

AU - Packham, Andrea E.

AU - Aguilar, Beatriz

AU - Conrad, Patricia A

PY - 2010/6

Y1 - 2010/6

N2 - To investigate how different routes of Toxoplasma gondii transmission influence the antibody response and infection status of deer mice (Peromyscus maniculatus), 80 mice were orally infected with 1, 5, 10, or 100 T. gondii oocysts. Ten weeks postinfection, 15 T. gondiiseropositive female mice were bred and allowed to produce 2 litters. Evidence of persistent T .gondii infection in orally infected mice was detected by serology and DNA amplification in mice from all 4 oocyst treatment groups, including those that received only a single T. gondii oocyst. Congenital transmission of T. gondii was detected by polymerase chain reaction (PCR) in 7/8 first and 4/7 second litters. Toxoplasma gondii was also detected by PCR in 9/30 congenitally infected offspring 16 wk after birth, despite the fact that detectable serological titers had waned. These findings raise questions about the applicability of serological testing to assess the prevalence of T. gondii infection in deer mice and other rodents in the wild. Additionally, the detection of frequent congenital transmission suggests that deer mice could help maintain T. gondii in the environment even in the absence of definitive feline hosts.

AB - To investigate how different routes of Toxoplasma gondii transmission influence the antibody response and infection status of deer mice (Peromyscus maniculatus), 80 mice were orally infected with 1, 5, 10, or 100 T. gondii oocysts. Ten weeks postinfection, 15 T. gondiiseropositive female mice were bred and allowed to produce 2 litters. Evidence of persistent T .gondii infection in orally infected mice was detected by serology and DNA amplification in mice from all 4 oocyst treatment groups, including those that received only a single T. gondii oocyst. Congenital transmission of T. gondii was detected by polymerase chain reaction (PCR) in 7/8 first and 4/7 second litters. Toxoplasma gondii was also detected by PCR in 9/30 congenitally infected offspring 16 wk after birth, despite the fact that detectable serological titers had waned. These findings raise questions about the applicability of serological testing to assess the prevalence of T. gondii infection in deer mice and other rodents in the wild. Additionally, the detection of frequent congenital transmission suggests that deer mice could help maintain T. gondii in the environment even in the absence of definitive feline hosts.

UR - http://www.scopus.com/inward/record.url?scp=77953852339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953852339&partnerID=8YFLogxK

U2 - 10.1645/GE-2372.1

DO - 10.1645/GE-2372.1

M3 - Article

C2 - 20557196

AN - SCOPUS:77953852339

VL - 96

SP - 516

EP - 520

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 3

ER -