Conformational studies of resin-bound vancomycin and the complex of vancomycin and Ac2-L-Lys-D-Ala-D-Ala

Nian Huan Yao, Wen Yi He, Kit Lam, Gang Liu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The molecular target of vancomycin, a commonly used glycopeptide antibiotic, is the D-Ala-D-Ala dipeptide subunit on the bacterial cell wall. The molecular basis of interaction between vancomycin and D-Ala-D-Ala in solution is well-known. However, there is no structural data on vancomycin, and its interaction with D-Ala-D-Ala when the drug is tethered to a solid support. In this Article, vancomycin was directly coupled onto TentaGel or PEGA resin through its C terminus. High-resolution magic angle spinning NMR studies indicated that conformation of PEGA bead-bound vancomycin is identical to that of the free drug. Broadening and shifts of the same proton resonances were observed in solution-phase vancomycin or PEGA-bound vancomycin when complexed with Ac2-L-Lys-D-Ala-D-Ala. This study demonstrates that bead-bound molecules can behave the same as solution-phase molecules in terms of molecular interaction with its target molecule, thus validating the on-bead screening approach of the "one-bead-one-compound" combinatorial library method.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalJournal of Combinatorial Chemistry
Volume7
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Vancomycin
Resins
Molecules
Drugs
Interaction
Target
Cell Wall
Antibiotics
Conformation
Screening
High Resolution
Angle
Magic angle spinning
Molecular interactions
Glycopeptides
Dipeptides
Demonstrate
N-(epsilon)-diacetyl-lysyl-alanyl-alanine N-(alpha)
Pharmaceutical Preparations
Conformations

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Discrete Mathematics and Combinatorics

Cite this

Conformational studies of resin-bound vancomycin and the complex of vancomycin and Ac2-L-Lys-D-Ala-D-Ala. / Yao, Nian Huan; He, Wen Yi; Lam, Kit; Liu, Gang.

In: Journal of Combinatorial Chemistry, Vol. 7, No. 1, 01.2005, p. 123-129.

Research output: Contribution to journalArticle

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