Conformational flexibility related to enzyme activity

Evidence for a dynamic active-site gatekeeper function of Tyr215 in Aerococcus viridans lactate oxidase

Thomas Stoisser, Michael Brunsteiner, David K. Wilson, Bernd Nidetzky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

L-Lactate oxidase (LOX) belongs to a large family of flavoenzymes that catalyze oxidation of α-hydroxy acids. How in these enzymes the protein structure controls reactivity presents an important but elusive problem. LOX contains a prominent tyrosine in the substrate binding pocket (Tyr215 in Aerococcus viridans LOX) that is partially responsible for securing a flexible loop which sequesters the active site. To characterize the role of Tyr215, effects of substitutions of the tyrosine (Y215F, Y215H) were analyzed kinetically, crystallographically and by molecular dynamics simulations. Enzyme variants showed slowed flavin reduction and oxidation by up to 33-fold. Pyruvate release was also decelerated and in Y215F, it was the slowest step overall. A 2.6-Å crystal structure of Y215F in complex with pyruvate shows the hydrogen bond between the phenolic hydroxyl and the keto oxygen in pyruvate is replaced with a potentially stronger hydrophobic interaction between the phenylalanine and the methyl group of pyruvate. Residues 200 through 215 or 216 appear to be disordered in two of the eight monomers in the asymmetric unit suggesting that they function as a lid controlling substrate entry and product exit from the active site. Substitutions of Tyr215 can thus lead to a kinetic bottleneck in product release.

Original languageEnglish (US)
Article number27892
JournalScientific Reports
Volume6
DOIs
StatePublished - Jun 15 2016

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lactate 2-monooxygenase
Enzyme activity
Pyruvic Acid
Tyrosine
Substitution reactions
Oxidation
Hydroxy Acids
Substrates
Enzymes
Hydroxyl Radical
Molecular dynamics
Hydrogen bonds
Monomers
Crystal structure
Oxygen
Kinetics
Computer simulation

ASJC Scopus subject areas

  • General

Cite this

Conformational flexibility related to enzyme activity : Evidence for a dynamic active-site gatekeeper function of Tyr215 in Aerococcus viridans lactate oxidase. / Stoisser, Thomas; Brunsteiner, Michael; Wilson, David K.; Nidetzky, Bernd.

In: Scientific Reports, Vol. 6, 27892, 15.06.2016.

Research output: Contribution to journalArticle

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