Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065

Andrew T M Vaughan, D. J. Grdina, P. J. Meechan, A. E. Milner, D. J. Gordon

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

WR 1065, 2-[(aminopropyl) amino] ethanethiol is an effective scavenger of free radicals. When present during irradiation it reduces cellular DNA damage as analysed by alkaline elution from filters. The same technique indicates that without irradiation, WR 1065 has no effect of DNA integrity. Using nucleoid analysis, where DNA damage is detected at the level of replicon clusters, WR 1065 distorts replicon supercoiling without breaking the DNA molecule. This confirmational change in nucleoid structure occurs with no detectable change in nucleoid protein content. It is proposed that perturbation of replicon supercoiling affects the process of normal DNA synthesis and strand break rejoining, allowing a longer time for the accurate repair of DNA damage.

Original languageEnglish (US)
Pages (from-to)893-896
Number of pages4
JournalBritish Journal of Cancer
Volume60
Issue number6
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Vaughan, A. T. M., Grdina, D. J., Meechan, P. J., Milner, A. E., & Gordon, D. J. (1989). Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065. British Journal of Cancer, 60(6), 893-896.