Conditional RNA interference mediated by allosteric ribozyme

Deepak Kumar, Chung Il An, Yohei Yokobayashi

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

(Figure Presented) Conditional RNA interference (RNAi) enables spatial and/or temporal control over gene silencing. The currently available methods require coexpression of engineered proteins and/or modified promoters which may limit their applications. We designed a novel RNA architecture that combines a drug-inducible allosteric ribozyme with a microRNA precursor analogue that allows chemical induction of RNAi in mammalian cells. The compact and highly modular RNA design should facilitate the construction of conditional RNAi systems that can sense and respond to a variety of molecules recognized by RNA aptamers to regulate virtually any desired genes sensitive to RNAi.

Original languageEnglish (US)
Pages (from-to)13906-13907
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number39
DOIs
StatePublished - Oct 7 2009

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Catalytic RNA
RNA Interference
RNA
Nucleotide Aptamers
Gene Silencing
Genes
MicroRNAs
Cells
Pharmaceutical Preparations
Proteins
Molecules

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Conditional RNA interference mediated by allosteric ribozyme. / Kumar, Deepak; An, Chung Il; Yokobayashi, Yohei.

In: Journal of the American Chemical Society, Vol. 131, No. 39, 07.10.2009, p. 13906-13907.

Research output: Contribution to journalArticle

Kumar, Deepak ; An, Chung Il ; Yokobayashi, Yohei. / Conditional RNA interference mediated by allosteric ribozyme. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 39. pp. 13906-13907.
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