Concentration-dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release

Paul S. Brookes, Emmanuel Padilla Salinas, Kenta Darley-Usmar, Jason P. Eiserich, Bruce A. Freeman, Victor M. Darley-Usmar, Peter G. Anderson

Research output: Contribution to journalArticle

257 Citations (Scopus)

Abstract

The mitochondrial permeability transition pore (PTP) and associated release of cytochrome c are thought to be important in the apoptotic process. Nitric oxide (NO·) has been reported to inhibit apoptosis by acting on a variety of extra-mitochondrial targets. The relationship between cytochrome c release and PTP opening, and the effects of NO· are not clearly established. Nitric oxide, S-nitrosothiols and peroxynitrite are reported to variously inhibit or promote PTP opening. In this study the effects of NO· on the PTP were characterized by exposing isolated rat liver mitochondria to physiological and pathological rates of NO· released from NONOate NO· donors. Nitric oxide reversibly inhibited PTP opening with an IC50 of 11 nM NO·/s, which can be readily achieved in vivo by NO· synthases. The mechanism involved mitochondrial membrane depolarization and inhibition of Ca2+ accumulation. At supraphysiological release rates (>2 μM/s) NO· accelerated PTP opening. Substantial cytochrome c release occurred with only a 20% change in mitochondrial swelling, was an early event in the PTP, and was also inhibited by NO·. Furthermore, NO· exposure resulted in significantly lower cytochrome c release for the same degree of PTP opening. It is proposed that this pathway represents an additional mechanism underlying the antiapoptotic effects of NO·.

Original languageEnglish (US)
Pages (from-to)20474-20479
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number27
DOIs
StatePublished - Jul 7 2000
Externally publishedYes

Fingerprint

Cytochromes c
Permeability
Nitric Oxide
S-Nitrosothiols
Mitochondrial Swelling
Mitochondria
Peroxynitrous Acid
Nitric Oxide Donors
Liver Mitochondrion
Depolarization
Mitochondrial Membranes
Nitric Oxide Synthase
Liver
Inhibitory Concentration 50
Swelling
Rats
Apoptosis
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Brookes, P. S., Padilla Salinas, E., Darley-Usmar, K., Eiserich, J. P., Freeman, B. A., Darley-Usmar, V. M., & Anderson, P. G. (2000). Concentration-dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release. Journal of Biological Chemistry, 275(27), 20474-20479. https://doi.org/10.1074/jbc.M001077200

Concentration-dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release. / Brookes, Paul S.; Padilla Salinas, Emmanuel; Darley-Usmar, Kenta; Eiserich, Jason P.; Freeman, Bruce A.; Darley-Usmar, Victor M.; Anderson, Peter G.

In: Journal of Biological Chemistry, Vol. 275, No. 27, 07.07.2000, p. 20474-20479.

Research output: Contribution to journalArticle

Brookes, PS, Padilla Salinas, E, Darley-Usmar, K, Eiserich, JP, Freeman, BA, Darley-Usmar, VM & Anderson, PG 2000, 'Concentration-dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release', Journal of Biological Chemistry, vol. 275, no. 27, pp. 20474-20479. https://doi.org/10.1074/jbc.M001077200
Brookes PS, Padilla Salinas E, Darley-Usmar K, Eiserich JP, Freeman BA, Darley-Usmar VM et al. Concentration-dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release. Journal of Biological Chemistry. 2000 Jul 7;275(27):20474-20479. https://doi.org/10.1074/jbc.M001077200
Brookes, Paul S. ; Padilla Salinas, Emmanuel ; Darley-Usmar, Kenta ; Eiserich, Jason P. ; Freeman, Bruce A. ; Darley-Usmar, Victor M. ; Anderson, Peter G. / Concentration-dependent effects of nitric oxide on mitochondrial permeability transition and cytochrome c release. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 27. pp. 20474-20479.
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