Computational analysis of contractility in engineered heart tissue

Grant Mathews, Claus Sondergaard, Angela Jeffreys, William Childs, Bao Linh Le, Amrit Sahota, Skender Najibi, Jan Nolta, Ming Sing Si

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Engineered heart tissue (EHT) is a potential therapy for heart failure and the basis of functional in vitro assays of novel cardiovascular treatments. Self-organizing EHT can be generated in fiber form, which makes the assessment of contractile function convenient with a force transducer. Contractile function is a key parameter of EHT performance. Analysis of EHT force data is often performed manually; however, this approach is time consuming, incomplete and subjective. Therefore, the purpose of this study was to develop a computer algorithm to efficiently and objectively analyze EHT force data. This algorithm incorporates data filtering, individual contraction detection and validation, inter/intracontractile analysis and intersample analysis. We found the algorithm to be accurate in contraction detection, validation and magnitude measurement as compared to human operators. The algorithm was efficient in processing hundreds of data acquisitions and was able to determine force-length curves, force-frequency relationships and compare various contractile parameters such as peak systolic force generation. We conclude that this computer algorithm is a key adjunct to the objective and efficient assessment of EHT contractile function.

Original languageEnglish (US)
Article number6156423
Pages (from-to)1429-1435
Number of pages7
JournalIEEE Transactions on Biomedical Engineering
Volume59
Issue number5
DOIs
StatePublished - May 2012

Keywords

  • Contraction
  • engineered heart tissue
  • force

ASJC Scopus subject areas

  • Biomedical Engineering

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    Mathews, G., Sondergaard, C., Jeffreys, A., Childs, W., Le, B. L., Sahota, A., Najibi, S., Nolta, J., & Si, M. S. (2012). Computational analysis of contractility in engineered heart tissue. IEEE Transactions on Biomedical Engineering, 59(5), 1429-1435. [6156423]. https://doi.org/10.1109/TBME.2012.2187899