Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice

Werner Welthagen, Robert A. Shellie, Joachim Spranger, Michael Ristow, Ralf Zimmermann, Oliver Fiehn

Research output: Contribution to journalArticle

130 Scopus citations

Abstract

Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF) was applied for the analysis of complex metabolite profiles from mouse spleen. The resulting two-dimensional chromatograms proved that mass spectral quality and sensitivity were largely improved by the enhanced resolution and zone compression, which are features of GC × GC operation, when compared to classical one-dimensional GC-TOF methods. The improved peak capacity of GC × GC allowed for peaks to be detected that could previously not be separated in one-dimensional GC. A measure of the combined power of chromatographic and mass spectral deconvolution resolution is called "analytical purity", with higher values indicating less pure peaks. GC × GC-TOF lead to the detection of 1200 compounds with purity better than 0.2, compared to 500 compounds with purity up to 2.5 in one-dimensional GC-TOF. The compounds identified include many of the compounds previously reported in NMR studies and other methods on mammalian tissues. Spleen samples of several obese NZO mice and lean C57BL/6 control strains were analyzed in order to demonstrate the applicability of GC × GC-TOF for biomarker identification.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalMetabolomics
Volume1
Issue number1
DOIs
StatePublished - Mar 2005

Keywords

  • Metabolic profiling
  • Metabonomics
  • Nutrigenomics
  • Obesity
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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