Comprehensive proteomic analysis of mesenchymal stem cell exosomes reveals modulation of angiogenesis via nuclear factor-kappaB signaling

Johnathon Anderson, Henrik J. Johansson, Calvin S. Graham, Mattias Vesterlund, Missy T. Pham, Charles S. Bramlett, Elizabeth N. Montgomery, Matt S. Mellema, Renee L. Bardini, Zelenia Contreras, Madeline Hoon, Gerhard Bauer, Kyle Fink, Brian Fury, Kyle J. Hendrix, Frederic Chedin, Samir El-Andaloussi, Billie Hwang, Michael S. Mulligan, Janne LehtiöJan Nolta

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSC) are known to facilitate healing of ischemic tissue related diseases through proangiogenic secretory proteins. Recent studies further show that MSC derived exosomes function as paracrine effectors of angiogenesis, however, the identity of which components of the exosome proteome responsible for this effect remains elusive. To address this we used high-resolution isoelectric focusing coupled liquid chromatography tandem mass spectrometry, an unbiased high throughput proteomics approach to comprehensively characterize the proteinaceous contents of MSCs and MSC derived exosomes. We probed the proteome of MSCs and MSC derived exosomes from cells cultured under expansion conditions and under ischemic tissue simulated conditions to elucidate key angiogenic paracrine effectors present and potentially differentially expressed in these conditions. In total, 6,342 proteins were identified in MSCs and 1,927 proteins in MSC derived exosomes, representing to our knowledge the first time these proteomes have been probed comprehensively. Multilayered analyses identified several putative paracrine effectors of angiogenesis present in MSC exosomes and increased in expression in MSCs exposed to ischemic tissue-simulated conditions; these include platelet derived growth factor, epidermal growth factor, fibroblast growth factor, and most notably nuclear factor-kappaB (NFkB) signaling pathway proteins. NFkB signaling was identified as a key mediator of MSC exosome induced angiogenesis in endothelial cells by functional in vitro validation using a specific inhibitor. Collectively, the results of our proteomic analysis show that MSC derived exosomes contain a robust profile of angiogenic paracrine effectors, which have potential for the treatment of ischemic tissue-related diseases.

Original languageEnglish (US)
Pages (from-to)601-613
Number of pages13
JournalStem Cells
Volume34
Issue number3
DOIs
StatePublished - Mar 1 2016

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Exosomes
Mesenchymal Stromal Cells
Proteomics
Proteome
Proteins
Fibroblast Growth Factors
Platelet-Derived Growth Factor
Isoelectric Focusing
Tandem Mass Spectrometry
Epidermal Growth Factor
Liquid Chromatography
Cultured Cells
Endothelial Cells

Keywords

  • Exosomes
  • High-resolution isoelectric focusing
  • Liquid chromatography tandem mass spectrometry
  • Mesenchymal stem cells
  • Nuclear factor kappaB
  • Peripheral arterial disease
  • Proteomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Comprehensive proteomic analysis of mesenchymal stem cell exosomes reveals modulation of angiogenesis via nuclear factor-kappaB signaling. / Anderson, Johnathon; Johansson, Henrik J.; Graham, Calvin S.; Vesterlund, Mattias; Pham, Missy T.; Bramlett, Charles S.; Montgomery, Elizabeth N.; Mellema, Matt S.; Bardini, Renee L.; Contreras, Zelenia; Hoon, Madeline; Bauer, Gerhard; Fink, Kyle; Fury, Brian; Hendrix, Kyle J.; Chedin, Frederic; El-Andaloussi, Samir; Hwang, Billie; Mulligan, Michael S.; Lehtiö, Janne; Nolta, Jan.

In: Stem Cells, Vol. 34, No. 3, 01.03.2016, p. 601-613.

Research output: Contribution to journalArticle

Anderson, J, Johansson, HJ, Graham, CS, Vesterlund, M, Pham, MT, Bramlett, CS, Montgomery, EN, Mellema, MS, Bardini, RL, Contreras, Z, Hoon, M, Bauer, G, Fink, K, Fury, B, Hendrix, KJ, Chedin, F, El-Andaloussi, S, Hwang, B, Mulligan, MS, Lehtiö, J & Nolta, J 2016, 'Comprehensive proteomic analysis of mesenchymal stem cell exosomes reveals modulation of angiogenesis via nuclear factor-kappaB signaling', Stem Cells, vol. 34, no. 3, pp. 601-613. https://doi.org/10.1002/stem.2298
Anderson, Johnathon ; Johansson, Henrik J. ; Graham, Calvin S. ; Vesterlund, Mattias ; Pham, Missy T. ; Bramlett, Charles S. ; Montgomery, Elizabeth N. ; Mellema, Matt S. ; Bardini, Renee L. ; Contreras, Zelenia ; Hoon, Madeline ; Bauer, Gerhard ; Fink, Kyle ; Fury, Brian ; Hendrix, Kyle J. ; Chedin, Frederic ; El-Andaloussi, Samir ; Hwang, Billie ; Mulligan, Michael S. ; Lehtiö, Janne ; Nolta, Jan. / Comprehensive proteomic analysis of mesenchymal stem cell exosomes reveals modulation of angiogenesis via nuclear factor-kappaB signaling. In: Stem Cells. 2016 ; Vol. 34, No. 3. pp. 601-613.
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AU - Pham, Missy T.

AU - Bramlett, Charles S.

AU - Montgomery, Elizabeth N.

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AU - Hendrix, Kyle J.

AU - Chedin, Frederic

AU - El-Andaloussi, Samir

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AU - Mulligan, Michael S.

AU - Lehtiö, Janne

AU - Nolta, Jan

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