Comprehensive genomic characterization of five canine lymphoid tumor cell lines

Sarah C. Roode, Daniel Rotroff, Kristy L. Richards, Peter F Moore, Alison Motsinger-Reif, Yasuhiko Okamura, Takuya Mizuno, Hajime Tsujimoto, Steven E. Suter, Matthew Breen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Leukemia/lymphoma cell lines have been critical in the investigation of the pathogenesis and therapy of hematological malignancies. While human LL cell lines have generally been found to recapitulate the primary tumors from which they were derived, appropriate characterization including cytogenetic and transcriptional assessment is crucial for assessing their clinical predictive value. Results: In the following study, five canine LL cell lines, CLBL-1, Ema, TL-1 (Nody-1), UL-1, and 3132, were characterized using extensive immunophenotyping, karyotypic analysis, oligonucleotide array comparative genomic hybridization (oaCGH), and gene expression profiling. Genome-wide DNA copy number data from the cell lines were also directly compared with 299 primary canine round cell tumors to determine whether the cell lines represent primary tumors, and, if so, what subtype each most closely resembled. Conclusions: Based on integrated analyses, CLBL-1 was classified as B-cell lymphoma, Ema and TL-1 as T-cell lymphoma, and UL-1 as T-cell acute lymphoblastic leukemia. 3132, originally classified as a B-cell lymphoma, was reclassified as a histiocytic sarcoma based on characteristic cytogenomic properties. In combination, these data begin to elucidate the clinical predictive value of these cell lines which will enhance the appropriate selection of in vitro models for future studies of canine hematological malignancies.

Original languageEnglish (US)
Article number207
JournalBMC Veterinary Research
Volume12
Issue number1
DOIs
StatePublished - Sep 17 2016

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Tumor Cell Line
Canidae
lymphoma
cell lines
Lymphocytes
genomics
Cell Line
dogs
neoplasms
B-lymphocytes
B-Cell Lymphoma
Hematologic Neoplasms
T-lymphocytes
comparative genomic hybridization
lymphocytic leukemia
Histiocytic Sarcoma
human cell lines
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
sarcoma
Immunophenotyping

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Roode, S. C., Rotroff, D., Richards, K. L., Moore, P. F., Motsinger-Reif, A., Okamura, Y., ... Breen, M. (2016). Comprehensive genomic characterization of five canine lymphoid tumor cell lines. BMC Veterinary Research, 12(1), [207]. https://doi.org/10.1186/s12917-016-0836-z

Comprehensive genomic characterization of five canine lymphoid tumor cell lines. / Roode, Sarah C.; Rotroff, Daniel; Richards, Kristy L.; Moore, Peter F; Motsinger-Reif, Alison; Okamura, Yasuhiko; Mizuno, Takuya; Tsujimoto, Hajime; Suter, Steven E.; Breen, Matthew.

In: BMC Veterinary Research, Vol. 12, No. 1, 207, 17.09.2016.

Research output: Contribution to journalArticle

Roode, SC, Rotroff, D, Richards, KL, Moore, PF, Motsinger-Reif, A, Okamura, Y, Mizuno, T, Tsujimoto, H, Suter, SE & Breen, M 2016, 'Comprehensive genomic characterization of five canine lymphoid tumor cell lines', BMC Veterinary Research, vol. 12, no. 1, 207. https://doi.org/10.1186/s12917-016-0836-z
Roode, Sarah C. ; Rotroff, Daniel ; Richards, Kristy L. ; Moore, Peter F ; Motsinger-Reif, Alison ; Okamura, Yasuhiko ; Mizuno, Takuya ; Tsujimoto, Hajime ; Suter, Steven E. ; Breen, Matthew. / Comprehensive genomic characterization of five canine lymphoid tumor cell lines. In: BMC Veterinary Research. 2016 ; Vol. 12, No. 1.
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