Abstract
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has undergone dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling.By reviewing 185 original papers and application notes, we can conclude that current advanced LC-MS-based lipidomics methods involve:. (1)lipid extraction schemes using chloroform/MeOH or methyl tert-butyl ether (MTBE)/MeOH, both with addition of internal standards covering each lipid class;(2)LC separation of lipids using short microbore C18 or C8 columns with sub-2-μm or 2.6-2.8-μm (fused-core) particle size with analysis time <30 min;(3)electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS.Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 192-206 |
| Number of pages | 15 |
| Journal | TrAC - Trends in Analytical Chemistry |
| Volume | 61 |
| DOIs | |
| State | Published - Oct 1 2014 |
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Keywords
- Acylglycerol
- Biological system
- Comprehensive analysis
- Extraction method
- Global lipidomic profiling
- LC-MS
- Lipidomics
- Liquid chromatography-mass spectrometry
- Metabolomics
- Phospholipid
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry. / Cajka, Tomas; Fiehn, Oliver.
In: TrAC - Trends in Analytical Chemistry, Vol. 61, 01.10.2014, p. 192-206.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry
AU - Cajka, Tomas
AU - Fiehn, Oliver
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has undergone dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling.By reviewing 185 original papers and application notes, we can conclude that current advanced LC-MS-based lipidomics methods involve:. (1)lipid extraction schemes using chloroform/MeOH or methyl tert-butyl ether (MTBE)/MeOH, both with addition of internal standards covering each lipid class;(2)LC separation of lipids using short microbore C18 or C8 columns with sub-2-μm or 2.6-2.8-μm (fused-core) particle size with analysis time <30 min;(3)electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS.Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.
AB - Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has undergone dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling.By reviewing 185 original papers and application notes, we can conclude that current advanced LC-MS-based lipidomics methods involve:. (1)lipid extraction schemes using chloroform/MeOH or methyl tert-butyl ether (MTBE)/MeOH, both with addition of internal standards covering each lipid class;(2)LC separation of lipids using short microbore C18 or C8 columns with sub-2-μm or 2.6-2.8-μm (fused-core) particle size with analysis time <30 min;(3)electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS.Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.
KW - Acylglycerol
KW - Biological system
KW - Comprehensive analysis
KW - Extraction method
KW - Global lipidomic profiling
KW - LC-MS
KW - Lipidomics
KW - Liquid chromatography-mass spectrometry
KW - Metabolomics
KW - Phospholipid
UR - http://www.scopus.com/inward/record.url?scp=84907015010&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907015010&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2014.04.017
DO - 10.1016/j.trac.2014.04.017
M3 - Article
VL - 61
SP - 192
EP - 206
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
SN - 0165-9936
ER -