Abstract
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has undergone dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling.By reviewing 185 original papers and application notes, we can conclude that current advanced LC-MS-based lipidomics methods involve:. (1)lipid extraction schemes using chloroform/MeOH or methyl tert-butyl ether (MTBE)/MeOH, both with addition of internal standards covering each lipid class;(2)LC separation of lipids using short microbore C18 or C8 columns with sub-2-μm or 2.6-2.8-μm (fused-core) particle size with analysis time <30 min;(3)electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS.Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.
Original language | English (US) |
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Pages (from-to) | 192-206 |
Number of pages | 15 |
Journal | TrAC - Trends in Analytical Chemistry |
Volume | 61 |
DOIs | |
State | Published - Oct 1 2014 |
Keywords
- Acylglycerol
- Biological system
- Comprehensive analysis
- Extraction method
- Global lipidomic profiling
- LC-MS
- Lipidomics
- Liquid chromatography-mass spectrometry
- Metabolomics
- Phospholipid
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry