Composition and characteristics of urinary calculi from guinea pigs

Michelle Hawkins, Annette L. Ruby, Tracy L. Drazenovich, Joellen L Westropp

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective-To determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis. Design-Cross-sectional study. Animals-127 guinea pigs. Procedures-Records of urinary calculi that had been submitted to the University of California Stone Laboratory from 1985 through 2003 were reviewed. In addition, submissions of urinary calculi for evaluation by the laboratory were prospectively solicited from 2004 through 2007. Prospectively obtained calculi were accompanied by a urine sample for urinalysis and bacteriologic culture and a completed questionnaire. All calculi were analyzed by use of polarized light microscopy and infrared spectroscopy. A subset of calculi was examined by means of x-ray diffractometry (XRD). Results-83% (43/52) of calculi from the laboratory database and 93% (70/75) of calculi that were prospectively solicited were composed of 100% calcium carbonate. Analysis via XRD confirmed that 5 of 6 calculi from a subset that had the greatest gross morphologic variation were composed of 100% calcite. Although many guinea pigs had received antimicrobials before bacteriologic cultures of urine were performed, Corynebacterium renale was isolated from 5 urine samples. Conclusions and Clinical Relevance-Contrary to findings of other studies, urinary calculi analyzed for the present study were most commonly composed of 100% calcium carbonate, and infrared spectroscopy or XRD was necessary to differentiate this mineral from others. Treatments, including diet and husbandry practices, should be developed to help prevent development of calcium carbonate calculi in guinea pigs.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalJournal of the American Veterinary Medical Association
Volume234
Issue number2
DOIs
StatePublished - Jan 15 2009

Fingerprint

urinary calculi
Urinary Calculi
calculi
Calculi
guinea pigs
Guinea Pigs
Calcium Carbonate
urine
calcium carbonate
Urine
X-radiation
Urinalysis
urinalysis
X-Rays
infrared spectroscopy
Minerals
Spectrum Analysis
Corynebacterium renale
Polarization Microscopy
Corynebacterium

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Composition and characteristics of urinary calculi from guinea pigs. / Hawkins, Michelle; Ruby, Annette L.; Drazenovich, Tracy L.; Westropp, Joellen L.

In: Journal of the American Veterinary Medical Association, Vol. 234, No. 2, 15.01.2009, p. 214-220.

Research output: Contribution to journalArticle

@article{b65649f1dd50427c935c0da2387cc244,
title = "Composition and characteristics of urinary calculi from guinea pigs",
abstract = "Objective-To determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis. Design-Cross-sectional study. Animals-127 guinea pigs. Procedures-Records of urinary calculi that had been submitted to the University of California Stone Laboratory from 1985 through 2003 were reviewed. In addition, submissions of urinary calculi for evaluation by the laboratory were prospectively solicited from 2004 through 2007. Prospectively obtained calculi were accompanied by a urine sample for urinalysis and bacteriologic culture and a completed questionnaire. All calculi were analyzed by use of polarized light microscopy and infrared spectroscopy. A subset of calculi was examined by means of x-ray diffractometry (XRD). Results-83{\%} (43/52) of calculi from the laboratory database and 93{\%} (70/75) of calculi that were prospectively solicited were composed of 100{\%} calcium carbonate. Analysis via XRD confirmed that 5 of 6 calculi from a subset that had the greatest gross morphologic variation were composed of 100{\%} calcite. Although many guinea pigs had received antimicrobials before bacteriologic cultures of urine were performed, Corynebacterium renale was isolated from 5 urine samples. Conclusions and Clinical Relevance-Contrary to findings of other studies, urinary calculi analyzed for the present study were most commonly composed of 100{\%} calcium carbonate, and infrared spectroscopy or XRD was necessary to differentiate this mineral from others. Treatments, including diet and husbandry practices, should be developed to help prevent development of calcium carbonate calculi in guinea pigs.",
author = "Michelle Hawkins and Ruby, {Annette L.} and Drazenovich, {Tracy L.} and Westropp, {Joellen L}",
year = "2009",
month = "1",
day = "15",
doi = "10.2460/javma.234.2.214",
language = "English (US)",
volume = "234",
pages = "214--220",
journal = "Journal of the American Veterinary Medical Association",
issn = "0003-1488",
publisher = "American Veterinary Medical Association",
number = "2",

}

TY - JOUR

T1 - Composition and characteristics of urinary calculi from guinea pigs

AU - Hawkins, Michelle

AU - Ruby, Annette L.

AU - Drazenovich, Tracy L.

AU - Westropp, Joellen L

PY - 2009/1/15

Y1 - 2009/1/15

N2 - Objective-To determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis. Design-Cross-sectional study. Animals-127 guinea pigs. Procedures-Records of urinary calculi that had been submitted to the University of California Stone Laboratory from 1985 through 2003 were reviewed. In addition, submissions of urinary calculi for evaluation by the laboratory were prospectively solicited from 2004 through 2007. Prospectively obtained calculi were accompanied by a urine sample for urinalysis and bacteriologic culture and a completed questionnaire. All calculi were analyzed by use of polarized light microscopy and infrared spectroscopy. A subset of calculi was examined by means of x-ray diffractometry (XRD). Results-83% (43/52) of calculi from the laboratory database and 93% (70/75) of calculi that were prospectively solicited were composed of 100% calcium carbonate. Analysis via XRD confirmed that 5 of 6 calculi from a subset that had the greatest gross morphologic variation were composed of 100% calcite. Although many guinea pigs had received antimicrobials before bacteriologic cultures of urine were performed, Corynebacterium renale was isolated from 5 urine samples. Conclusions and Clinical Relevance-Contrary to findings of other studies, urinary calculi analyzed for the present study were most commonly composed of 100% calcium carbonate, and infrared spectroscopy or XRD was necessary to differentiate this mineral from others. Treatments, including diet and husbandry practices, should be developed to help prevent development of calcium carbonate calculi in guinea pigs.

AB - Objective-To determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis. Design-Cross-sectional study. Animals-127 guinea pigs. Procedures-Records of urinary calculi that had been submitted to the University of California Stone Laboratory from 1985 through 2003 were reviewed. In addition, submissions of urinary calculi for evaluation by the laboratory were prospectively solicited from 2004 through 2007. Prospectively obtained calculi were accompanied by a urine sample for urinalysis and bacteriologic culture and a completed questionnaire. All calculi were analyzed by use of polarized light microscopy and infrared spectroscopy. A subset of calculi was examined by means of x-ray diffractometry (XRD). Results-83% (43/52) of calculi from the laboratory database and 93% (70/75) of calculi that were prospectively solicited were composed of 100% calcium carbonate. Analysis via XRD confirmed that 5 of 6 calculi from a subset that had the greatest gross morphologic variation were composed of 100% calcite. Although many guinea pigs had received antimicrobials before bacteriologic cultures of urine were performed, Corynebacterium renale was isolated from 5 urine samples. Conclusions and Clinical Relevance-Contrary to findings of other studies, urinary calculi analyzed for the present study were most commonly composed of 100% calcium carbonate, and infrared spectroscopy or XRD was necessary to differentiate this mineral from others. Treatments, including diet and husbandry practices, should be developed to help prevent development of calcium carbonate calculi in guinea pigs.

UR - http://www.scopus.com/inward/record.url?scp=59049097576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59049097576&partnerID=8YFLogxK

U2 - 10.2460/javma.234.2.214

DO - 10.2460/javma.234.2.214

M3 - Article

C2 - 19210239

AN - SCOPUS:59049097576

VL - 234

SP - 214

EP - 220

JO - Journal of the American Veterinary Medical Association

JF - Journal of the American Veterinary Medical Association

SN - 0003-1488

IS - 2

ER -