Complex i-associated hydrogen peroxide production is decreased and electron transport chain enzyme activities are altered in n-3 enriched fat-1 mice

Kevork Hagopian, Kristina L. Weber, Darren T. Hwee, Alison L. van Eenennaam, Guillermo López-Lluch, José M. Villalba, Isabel Burón, Plácido Navas, J. Bruce German, Steven M. Watkins, Yana Chen, Alfreda Wei, Roger B. McDonald, Jon J Ramsey

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The polyunsaturated nature of n-3 fatty acids makes them prone to oxidative damage. However, it is not clear if n-3 fatty acids are simply a passive site for oxidative attack or if they also modulate mitochondrial reactive oxygen species (ROS) production. The present study used fat-1 transgenic mice, that are capable of synthesizing n-3 fatty acids, to investigate the influence of increases in n-3 fatty acids and resultant decreases in the n-6:n-3 ratio on liver mitochondrial H2O2 production and electron transport chain (ETC) activity. There was an increase in n-3 fatty acids and a decrease in the n-6:n-3 ratio in liver mitochondria from the fat-1 compared to control mice. This change was largely due to alterations in the fatty acid composition of phosphatidylcholine and phosphatidylethanolamine, with only a small percentage of fatty acids in cardiolipin being altered in the fat-1 animals. The lipid changes in the fat-1 mice were associated with a decrease (p<0.05) in the activity of ETC complex I and increases (p<0.05) in the activities of complexes III and IV. Mitochondrial H2O2 production with either succinate or succinate/glutamate/malate substrates was also decreased (p<0.05) in the fat-1 mice. This change in H2O2 production was due to a decrease in ROS production from ETC complex I in the fat-1 animals. These results indicate that the fatty acid changes in fat-1 liver mitochondria may at least partially oppose oxidative stress by limiting ROS production from ETC complex I.

Original languageEnglish (US)
Article numbere12696
Pages (from-to)1-11
Number of pages11
JournalPLoS One
Volume5
Issue number9
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Complex i-associated hydrogen peroxide production is decreased and electron transport chain enzyme activities are altered in n-3 enriched fat-1 mice'. Together they form a unique fingerprint.

  • Cite this

    Hagopian, K., Weber, K. L., Hwee, D. T., van Eenennaam, A. L., López-Lluch, G., Villalba, J. M., Burón, I., Navas, P., German, J. B., Watkins, S. M., Chen, Y., Wei, A., McDonald, R. B., & Ramsey, J. J. (2010). Complex i-associated hydrogen peroxide production is decreased and electron transport chain enzyme activities are altered in n-3 enriched fat-1 mice. PLoS One, 5(9), 1-11. [e12696]. https://doi.org/10.1371/journal.pone.0012696