Complex conjugate artifact-free adaptive optics optical coherence tomography of in vivo human optic nerve head

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9 Scopus citations


We acquired in vivo images of the human optic nerve head (ONH) using an adaptive optics-optical coherence tomography (AO-OCT) system. In order to improve imaging of the lamina cribrosa in the ONH with high lateral resolution and sensitivity, we implemented a complex conjugate artifact-free Fourier domain OCT (Fd-OCT) acquisition scheme with a reference arm-based phase shifting method. This allowed positioning of the lamina cribrosa structures near the zero path length difference where AO-OCT imaging achieves highest sensitivity. Implementation of our complex conjugate artifact removal (CCR) method required constant phase shifts between consecutive axial scans (A-scans), generated by continuous beam path-length changes from offsetting the pivot point of the scanning mirror placed in the reference arm. Fourier transform along the transverse axis and a filtering algorithm allowed reconstruction of CCR AO-OCT images. The suppression ratio of the mirror artifact was approximately 22 dB (at 18,000 A-scans per second acquisition speed) with a paperboard test target and an optimum phase-shift value. Finally, we reconstructed the three-dimensional structure of human ONH with enhanced depth range and sensitivity using CCR AO-OCT.

Original languageEnglish (US)
Article number126005
JournalJournal of Biomedical Optics
Issue number12
StatePublished - Dec 2012


  • Aberration compensation
  • Adaptive optics
  • Complex conjugate artifact
  • Imaging systems
  • Medical optics instrumentation
  • Ophthalmology
  • Optical coherence tomography

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


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