Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene

Elise A. Rose, Thomas M Glaser, Carol Jones, Cassandra L. Smith, William H. Lewis, Katherine M. Call, Mark Minden, Eric Champagne, Laura Bonetta, Herman Yeger, David E. Housman

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

A complete physical map of the 11p13 region containing the Wilms' tumor locus has been developed and used to localize a candidate Wilms' tumor gene. Our strategy to construct the map combined the use of pulsed-field gel electrophoresis and irradiation-reduced somatic cell hybrids. These hybrids, which contain limited segments of human chromosome 11 segregrated from the remainder of the human genome, permit direct visualization of restriction fragments located in 11p13 using human interspersed repeated DNA sequences as hybridization probes. The physical map has provided a framework to identify the sites of genes responsible for the complex of disorders associated with hemizygous 11p13 deletion: Wilms' tumor, aniridia, genitourinary abnormalities, and mental retardation. The Wilms' tumor locus has been limited to a region of less than 345 kb, and a transcript with many of the characteristics expected for the Wilms' tumor gene has been localized to this region.

Original languageEnglish (US)
Pages (from-to)495-508
Number of pages14
JournalCell
Volume60
Issue number3
DOIs
StatePublished - Feb 9 1990
Externally publishedYes

Fingerprint

Wilms' Tumor Genes
Wilms Tumor
Tumors
Genes
Urogenital Abnormalities
Aniridia
Chromosomes, Human, Pair 11
Hybrid Cells
Pulsed Field Gel Electrophoresis
Human Chromosomes
Human Genome
Intellectual Disability
DNA sequences
Chromosomes
Electrophoresis
Visualization
Gels
Irradiation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Rose, E. A., Glaser, T. M., Jones, C., Smith, C. L., Lewis, W. H., Call, K. M., ... Housman, D. E. (1990). Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene. Cell, 60(3), 495-508. https://doi.org/10.1016/0092-8674(90)90600-J

Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene. / Rose, Elise A.; Glaser, Thomas M; Jones, Carol; Smith, Cassandra L.; Lewis, William H.; Call, Katherine M.; Minden, Mark; Champagne, Eric; Bonetta, Laura; Yeger, Herman; Housman, David E.

In: Cell, Vol. 60, No. 3, 09.02.1990, p. 495-508.

Research output: Contribution to journalArticle

Rose, EA, Glaser, TM, Jones, C, Smith, CL, Lewis, WH, Call, KM, Minden, M, Champagne, E, Bonetta, L, Yeger, H & Housman, DE 1990, 'Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene', Cell, vol. 60, no. 3, pp. 495-508. https://doi.org/10.1016/0092-8674(90)90600-J
Rose, Elise A. ; Glaser, Thomas M ; Jones, Carol ; Smith, Cassandra L. ; Lewis, William H. ; Call, Katherine M. ; Minden, Mark ; Champagne, Eric ; Bonetta, Laura ; Yeger, Herman ; Housman, David E. / Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene. In: Cell. 1990 ; Vol. 60, No. 3. pp. 495-508.
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