Complete genome sequence of the thermophilic, hydrogen-oxidizing Bacillus tusciae type strain (T2 T) and reclassification in the new genus, Kyrpidia gen. nov. as Kyrpidia tusciae comb. nov. and emendation of the family alicyclobacillaceae da Costa and Rainey, 2010

Hans Peter Klenk, Alla Lapidus, Olga Chertkov, Alex Copeland, Glavina Tijana del Rio, Matt Nolan, Susan Lucas, Feng Chen, Hope Tice, Jan Fang Cheng, Cliff Han, David Bruce, Lynne Goodwin, Sam Pitluck, Amrita Pati, Natalia Ivanova, Konstantinos Mavromatis, Chris Daum, Amy Chen, Krishna PalaniappanYun Juan Chang, Miriam Land, Loren Hauser, Cynthia D. Jeffries, John C. Detter, Manfred Rohde, Birte Abt, Rüdiger Pukall, Markus Göker, James Bristow, Victor Markowitz, Philip Hugenholtz, Jonathan A Eisen

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Bacillus tusciae Bonjour & Aragno 1994 is a hydrogen-oxidizing, thermoacidophilic spore former that lives as a facultative chemolithoautotroph in solfataras. Although 16S rRNA gene sequencing was well established at the time of the initial description of the organism, 16S sequence data were not available and the strain was placed into the genus Bacillus based on limited chemotaxonomic information. Despite the now obvious misplacement of strain T2 as a member of the genus Bacillus in 16S rRNA-based phylogenetic trees, the misclassification remained uncorrected for many years, which was likely due to the extremely difficult, analysis-hampering cultivation conditions and poor growth rate of the strain. Here we provide a taxonomic re-evaluation of strain T2T (= DSM 2912 = NBRC 15312) and propose its reclassification as the type strain of a new species, Kyrpidia tusciae, and the type species of the new genus Kyrpidia, which is a sister-group of Alicyclobacillus. The family Alicyclobacillaceae da Costa and Rainey, 2010 is emended. The 3,384,766 bp genome with its 3,323 protein-coding and 78 RNA genes is part of the GenomicEncyclopedia ofBacteria andArchaea project.

Original languageEnglish (US)
JournalStandards in Genomic Sciences
Volume5
Issue number1
DOIs
StatePublished - 2011

Keywords

  • Aerobe
  • Bacillaceae
  • Facultative chemolithoautotroph
  • Free-living
  • GEBA
  • Hydrogen-oxidizing
  • Solfatara
  • Spore-forming
  • Thermoacidophile

ASJC Scopus subject areas

  • Genetics

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    Klenk, H. P., Lapidus, A., Chertkov, O., Copeland, A., del Rio, G. T., Nolan, M., Lucas, S., Chen, F., Tice, H., Cheng, J. F., Han, C., Bruce, D., Goodwin, L., Pitluck, S., Pati, A., Ivanova, N., Mavromatis, K., Daum, C., Chen, A., ... Eisen, J. A. (2011). Complete genome sequence of the thermophilic, hydrogen-oxidizing Bacillus tusciae type strain (T2 T) and reclassification in the new genus, Kyrpidia gen. nov. as Kyrpidia tusciae comb. nov. and emendation of the family alicyclobacillaceae da Costa and Rainey, 2010. Standards in Genomic Sciences, 5(1). https://doi.org/10.4056/sigs.2144922