Complete genome sequence of the Q-fever pathogen Coxiella burnetii

Rekha Seshadri, Ian T. Paulsen, Jonathan A Eisen, Timothy D. Read, Karen E. Nelson, William C. Nelson, Naomi L. Ward, Hervé Tettelin, Tanja M. Davidsen, Maureen J. Beanan, Robert T. Deboy, Sean C. Daugherty, Lauren M. Brinkac, Ramana Madupu, Robert J. Dodson, Hoda M. Khouri, Kathy H. Lee, Heather A. Carty, David Scanlan, Robert A. HeinzenHerbert A. Thompson, James E. Samuel, Claire M. Fraser, John F. Heidelberg

Research output: Contribution to journalArticle

365 Citations (Scopus)

Abstract

The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This bacterium is an obligate intracellular acidophile that is highly adapted for life within the eukaryotic phagolysosome. Genome analysis revealed many genes with potential roles in adhesion, invasion, intracellular trafficking, host-cell modulation, and detoxification. A previously uncharacterized 13-member family of ankyrin repeat-containing proteins is implicated in the pathogenesis of this organism. Although the lifestyle and parasitic strategies of C. burnetii resemble that of Rickettsiae and Chlamydiae, their genome architectures differ considerably in terms of presence of mobile elements, extent of genome reduction, metabolic capabilities, and transporter profiles. The presence of 83 pseudogenes displays an ongoing process of gene degradation. Unlike other obligate intracellular bacteria, 32 insertion sequences are found dispersed in the chromosome, indicating some plasticity in the C. burnetii genome. These analyses suggest that the obligate intracellular lifestyle of C. burnetii may be a relatively recent innovation.

Original languageEnglish (US)
Pages (from-to)5455-5460
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number9
DOIs
StatePublished - Apr 29 2003
Externally publishedYes

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Coxiella burnetii
Q Fever
Genome
Life Style
Biological Warfare Agents
Ankyrin Repeat
Bacteria
Rickettsia
Phagosomes
Pseudogenes
Chlamydia
Insertional Mutagenesis
Zoonoses
Firearms
Genes
Chromosomes
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Complete genome sequence of the Q-fever pathogen Coxiella burnetii. / Seshadri, Rekha; Paulsen, Ian T.; Eisen, Jonathan A; Read, Timothy D.; Nelson, Karen E.; Nelson, William C.; Ward, Naomi L.; Tettelin, Hervé; Davidsen, Tanja M.; Beanan, Maureen J.; Deboy, Robert T.; Daugherty, Sean C.; Brinkac, Lauren M.; Madupu, Ramana; Dodson, Robert J.; Khouri, Hoda M.; Lee, Kathy H.; Carty, Heather A.; Scanlan, David; Heinzen, Robert A.; Thompson, Herbert A.; Samuel, James E.; Fraser, Claire M.; Heidelberg, John F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 9, 29.04.2003, p. 5455-5460.

Research output: Contribution to journalArticle

Seshadri, R, Paulsen, IT, Eisen, JA, Read, TD, Nelson, KE, Nelson, WC, Ward, NL, Tettelin, H, Davidsen, TM, Beanan, MJ, Deboy, RT, Daugherty, SC, Brinkac, LM, Madupu, R, Dodson, RJ, Khouri, HM, Lee, KH, Carty, HA, Scanlan, D, Heinzen, RA, Thompson, HA, Samuel, JE, Fraser, CM & Heidelberg, JF 2003, 'Complete genome sequence of the Q-fever pathogen Coxiella burnetii', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 9, pp. 5455-5460. https://doi.org/10.1073/pnas.0931379100
Seshadri, Rekha ; Paulsen, Ian T. ; Eisen, Jonathan A ; Read, Timothy D. ; Nelson, Karen E. ; Nelson, William C. ; Ward, Naomi L. ; Tettelin, Hervé ; Davidsen, Tanja M. ; Beanan, Maureen J. ; Deboy, Robert T. ; Daugherty, Sean C. ; Brinkac, Lauren M. ; Madupu, Ramana ; Dodson, Robert J. ; Khouri, Hoda M. ; Lee, Kathy H. ; Carty, Heather A. ; Scanlan, David ; Heinzen, Robert A. ; Thompson, Herbert A. ; Samuel, James E. ; Fraser, Claire M. ; Heidelberg, John F. / Complete genome sequence of the Q-fever pathogen Coxiella burnetii. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 9. pp. 5455-5460.
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