Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83

Karen E. Nelson, Robert D. Fleischmann, Robert T. DeBoy, Ian T. Paulsen, Derrick E. Fouts, Jonathan A Eisen, Sean C. Daugherty, Robert J. Dodson, A. Scott Durkin, Michelle Gwinn, Daniel H. Haft, James F. Kolonay, William C. Nelson, Tanya Mason, Luke Tallon, Jessica Gray, David Granger, Hervé Tettelin, Hong Dong, Jamie L. GalvinMargaret J. Duncan, Floyd E. Dewhirst, Claire M. Fraser

Research output: Contribution to journalArticle

273 Citations (Scopus)

Abstract

The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.

Original languageEnglish (US)
Pages (from-to)5591-5601
Number of pages11
JournalJournal of Bacteriology
Volume185
Issue number18
DOIs
StatePublished - Sep 2003
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Cytophaga
Flavobacterium
Genome
Bacteroides
Bacteria
Periodontal Diseases
Chlorobium
Chlorobi
Poisons
Hemagglutinins
Virulence
Peptide Hydrolases
Amino Acids
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Nelson, K. E., Fleischmann, R. D., DeBoy, R. T., Paulsen, I. T., Fouts, D. E., Eisen, J. A., ... Fraser, C. M. (2003). Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83. Journal of Bacteriology, 185(18), 5591-5601. https://doi.org/10.1128/JB.185.18.5591-5601.2003

Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83. / Nelson, Karen E.; Fleischmann, Robert D.; DeBoy, Robert T.; Paulsen, Ian T.; Fouts, Derrick E.; Eisen, Jonathan A; Daugherty, Sean C.; Dodson, Robert J.; Durkin, A. Scott; Gwinn, Michelle; Haft, Daniel H.; Kolonay, James F.; Nelson, William C.; Mason, Tanya; Tallon, Luke; Gray, Jessica; Granger, David; Tettelin, Hervé; Dong, Hong; Galvin, Jamie L.; Duncan, Margaret J.; Dewhirst, Floyd E.; Fraser, Claire M.

In: Journal of Bacteriology, Vol. 185, No. 18, 09.2003, p. 5591-5601.

Research output: Contribution to journalArticle

Nelson, KE, Fleischmann, RD, DeBoy, RT, Paulsen, IT, Fouts, DE, Eisen, JA, Daugherty, SC, Dodson, RJ, Durkin, AS, Gwinn, M, Haft, DH, Kolonay, JF, Nelson, WC, Mason, T, Tallon, L, Gray, J, Granger, D, Tettelin, H, Dong, H, Galvin, JL, Duncan, MJ, Dewhirst, FE & Fraser, CM 2003, 'Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83', Journal of Bacteriology, vol. 185, no. 18, pp. 5591-5601. https://doi.org/10.1128/JB.185.18.5591-5601.2003
Nelson, Karen E. ; Fleischmann, Robert D. ; DeBoy, Robert T. ; Paulsen, Ian T. ; Fouts, Derrick E. ; Eisen, Jonathan A ; Daugherty, Sean C. ; Dodson, Robert J. ; Durkin, A. Scott ; Gwinn, Michelle ; Haft, Daniel H. ; Kolonay, James F. ; Nelson, William C. ; Mason, Tanya ; Tallon, Luke ; Gray, Jessica ; Granger, David ; Tettelin, Hervé ; Dong, Hong ; Galvin, Jamie L. ; Duncan, Margaret J. ; Dewhirst, Floyd E. ; Fraser, Claire M. / Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 18. pp. 5591-5601.
@article{9f997de288164e5c9f5e16adf72018f9,
title = "Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83",
abstract = "The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.",
author = "Nelson, {Karen E.} and Fleischmann, {Robert D.} and DeBoy, {Robert T.} and Paulsen, {Ian T.} and Fouts, {Derrick E.} and Eisen, {Jonathan A} and Daugherty, {Sean C.} and Dodson, {Robert J.} and Durkin, {A. Scott} and Michelle Gwinn and Haft, {Daniel H.} and Kolonay, {James F.} and Nelson, {William C.} and Tanya Mason and Luke Tallon and Jessica Gray and David Granger and Herv{\'e} Tettelin and Hong Dong and Galvin, {Jamie L.} and Duncan, {Margaret J.} and Dewhirst, {Floyd E.} and Fraser, {Claire M.}",
year = "2003",
month = "9",
doi = "10.1128/JB.185.18.5591-5601.2003",
language = "English (US)",
volume = "185",
pages = "5591--5601",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "18",

}

TY - JOUR

T1 - Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83

AU - Nelson, Karen E.

AU - Fleischmann, Robert D.

AU - DeBoy, Robert T.

AU - Paulsen, Ian T.

AU - Fouts, Derrick E.

AU - Eisen, Jonathan A

AU - Daugherty, Sean C.

AU - Dodson, Robert J.

AU - Durkin, A. Scott

AU - Gwinn, Michelle

AU - Haft, Daniel H.

AU - Kolonay, James F.

AU - Nelson, William C.

AU - Mason, Tanya

AU - Tallon, Luke

AU - Gray, Jessica

AU - Granger, David

AU - Tettelin, Hervé

AU - Dong, Hong

AU - Galvin, Jamie L.

AU - Duncan, Margaret J.

AU - Dewhirst, Floyd E.

AU - Fraser, Claire M.

PY - 2003/9

Y1 - 2003/9

N2 - The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.

AB - The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.

UR - http://www.scopus.com/inward/record.url?scp=0041335299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041335299&partnerID=8YFLogxK

U2 - 10.1128/JB.185.18.5591-5601.2003

DO - 10.1128/JB.185.18.5591-5601.2003

M3 - Article

C2 - 12949112

AN - SCOPUS:0041335299

VL - 185

SP - 5591

EP - 5601

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 18

ER -