Complete genome sequence of Caulobacter crescentus

William C. Nierman, Tamara V. Feldblyum, Michael T. Laub, Ian T. Paulsen, Karen E. Nelson, Jonathan A Eisen, John F. Heidelberg, M. R K Alley, Noriko Ohta, Janine R. Maddock, Isabel Potocka, William C. Nelson, Austin Newton, Craig Stephens, Nikhil D. Phadke, Bert Ely, Robert T. DeBoy, Robert J. Dodson, A. Scott Durkin, Michelle L. GwinnDaniel H. Haft, James F. Kolonay, John Smit, M. B. Craven, Hoda Khouri, Jyoti Shetty, Kristi Berry, Teresa Utterback, Kevin Tran, Alex Wolf, Jessica Vamathevan, Maria Ermolaeva, Owen White, Steven L. Salzberg, J. Craig Venter, Lucy Shapiro, Claire M. Fraser

Research output: Contribution to journalArticle

389 Citations (Scopus)

Abstract

The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. This organism, which grows in a dilute aquatic environment, coordinates the cell division cycle and multiple cell differentiation events. With the annotated genome sequence, a full description of the genetic network that controls bacterial differentiation, cell growth, and cell cycle progression is within reach. Two-component signal transduction proteins are known to play a significant role in cell cycle progression. Genome analysis revealed that the C. crescentus genome encodes a significantly higher number of these signaling proteins (105) than any bacterial genome sequenced thus far. Another regulatory mechanism involved in cell cycle progression is DNA methylation. The occurrence of the recognition sequence for an essential DNA methylating enzyme that is required for cell cycle regulation is severely limited and shows a bias to intergenic regions. The genome contains multiple clusters of genes encoding proteins essential for survival in a nutrient poor habitat. Included are those involved in chemotaxis, outer membrane channel function, degradation of aromatic ring compounds, and the breakdown of plant-derived carbon sources, in addition to many extracytoplasmic function sigma factors, providing the organism with the ability to respond to a wide range of environmental fluctuations. C. crescentus is, to our knowledge, the first free-living α-class proteobacterium to be sequenced and will serve as a foundation for exploring the biology of this group of bacteria, which includes the obligate endosymbiont and human pathogen Rickettsia prowazekii, the plant pathogen Agrobacterium tumefaciens, and the bovine and human pathogen Brucella abortus.

Original languageEnglish (US)
Pages (from-to)4136-4141
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number7
DOIs
StatePublished - Mar 27 2001
Externally publishedYes

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Caulobacter crescentus
Cell Cycle
Genome
Cell Differentiation
Rickettsia prowazekii
Bacterial Genomes
Brucella abortus
Sigma Factor
Proteobacteria
Agrobacterium tumefaciens
Proteins
Intergenic DNA
Chromosomes, Human, Pair 3
Aptitude
Phytochemicals
Chemotaxis
DNA Methylation
Ion Channels
Base Pairing
Ecosystem

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Nierman, W. C., Feldblyum, T. V., Laub, M. T., Paulsen, I. T., Nelson, K. E., Eisen, J. A., ... Fraser, C. M. (2001). Complete genome sequence of Caulobacter crescentus. Proceedings of the National Academy of Sciences of the United States of America, 98(7), 4136-4141. https://doi.org/10.1073/pnas.061029298

Complete genome sequence of Caulobacter crescentus. / Nierman, William C.; Feldblyum, Tamara V.; Laub, Michael T.; Paulsen, Ian T.; Nelson, Karen E.; Eisen, Jonathan A; Heidelberg, John F.; Alley, M. R K; Ohta, Noriko; Maddock, Janine R.; Potocka, Isabel; Nelson, William C.; Newton, Austin; Stephens, Craig; Phadke, Nikhil D.; Ely, Bert; DeBoy, Robert T.; Dodson, Robert J.; Durkin, A. Scott; Gwinn, Michelle L.; Haft, Daniel H.; Kolonay, James F.; Smit, John; Craven, M. B.; Khouri, Hoda; Shetty, Jyoti; Berry, Kristi; Utterback, Teresa; Tran, Kevin; Wolf, Alex; Vamathevan, Jessica; Ermolaeva, Maria; White, Owen; Salzberg, Steven L.; Venter, J. Craig; Shapiro, Lucy; Fraser, Claire M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 7, 27.03.2001, p. 4136-4141.

Research output: Contribution to journalArticle

Nierman, WC, Feldblyum, TV, Laub, MT, Paulsen, IT, Nelson, KE, Eisen, JA, Heidelberg, JF, Alley, MRK, Ohta, N, Maddock, JR, Potocka, I, Nelson, WC, Newton, A, Stephens, C, Phadke, ND, Ely, B, DeBoy, RT, Dodson, RJ, Durkin, AS, Gwinn, ML, Haft, DH, Kolonay, JF, Smit, J, Craven, MB, Khouri, H, Shetty, J, Berry, K, Utterback, T, Tran, K, Wolf, A, Vamathevan, J, Ermolaeva, M, White, O, Salzberg, SL, Venter, JC, Shapiro, L & Fraser, CM 2001, 'Complete genome sequence of Caulobacter crescentus', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 7, pp. 4136-4141. https://doi.org/10.1073/pnas.061029298
Nierman, William C. ; Feldblyum, Tamara V. ; Laub, Michael T. ; Paulsen, Ian T. ; Nelson, Karen E. ; Eisen, Jonathan A ; Heidelberg, John F. ; Alley, M. R K ; Ohta, Noriko ; Maddock, Janine R. ; Potocka, Isabel ; Nelson, William C. ; Newton, Austin ; Stephens, Craig ; Phadke, Nikhil D. ; Ely, Bert ; DeBoy, Robert T. ; Dodson, Robert J. ; Durkin, A. Scott ; Gwinn, Michelle L. ; Haft, Daniel H. ; Kolonay, James F. ; Smit, John ; Craven, M. B. ; Khouri, Hoda ; Shetty, Jyoti ; Berry, Kristi ; Utterback, Teresa ; Tran, Kevin ; Wolf, Alex ; Vamathevan, Jessica ; Ermolaeva, Maria ; White, Owen ; Salzberg, Steven L. ; Venter, J. Craig ; Shapiro, Lucy ; Fraser, Claire M. / Complete genome sequence of Caulobacter crescentus. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 7. pp. 4136-4141.
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AU - Eisen, Jonathan A

AU - Heidelberg, John F.

AU - Alley, M. R K

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AU - DeBoy, Robert T.

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AU - Durkin, A. Scott

AU - Gwinn, Michelle L.

AU - Haft, Daniel H.

AU - Kolonay, James F.

AU - Smit, John

AU - Craven, M. B.

AU - Khouri, Hoda

AU - Shetty, Jyoti

AU - Berry, Kristi

AU - Utterback, Teresa

AU - Tran, Kevin

AU - Wolf, Alex

AU - Vamathevan, Jessica

AU - Ermolaeva, Maria

AU - White, Owen

AU - Salzberg, Steven L.

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AU - Shapiro, Lucy

AU - Fraser, Claire M.

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