Complete genome sequence of a virulent isolate of Streptococcus pneumoniae

H. Tettelin, K. E. Nelson, I. T. Paulsen, Jonathan A Eisen, T. D. Read, S. Peterson, J. Heidelberg, R. T. DeBoy, D. H. Haft, R. J. Dodson, A. S. Durkin, M. Gwinn, J. F. Kolonay, W. C. Nelson, J. D. Peterson, L. A. Umayam, O. White, S. L. Salzberg, M. R. Lewis, D. RaduneE. Holtzapple, H. Khouri, A. M. Wolf, T. R. Utterback, C. L. Hansen, L. A. McDonald, T. V. Feldblyum, S. Angiuoli, T. Dickinson, E. K. Hickey, I. E. Holt, B. J. Loftus, F. Yang, H. O. Smith, J. C. Venter, B. A. Dougherty, D. A. Morrison, S. K. Hollingshead, C. M. Fraser

Research output: Contribution to journalArticle

910 Citations (Scopus)

Abstract

The 2,160,837-base pair genome sequence of an isolate of Streptococcus pneumoniae, a Gram-positive pathogen that causes pneumonia, bacteremia, meningitis, and otitis media, contains 2236 predicted coding regions; of these, 1440 (64%) were assigned a biological role. Approximately 5% of the genome is composed of insertion sequences that may contribute to genome rearrangements through uptake of foreign DNA. Extracellular enzyme systems for the metabolism of polysaccharides and hexosamines provide a substantial source of carbon and nitrogen for S. pneumoniae and also damage host tissues and facilitate colonization. A motif identified within the signal peptide of proteins is potentially involved in targeting these proteins to the cell surface of low-guanine/cytosine (GC) Gram-positive species. Several surface-exposed proteins that may serve as potential vaccine candidates were identified. Comparative genome hybridization with DNA arrays revealed strain differences in S. pneumoniae that could contribute to differences in virulence and antigenicity.

Original languageEnglish (US)
Pages (from-to)498-506
Number of pages9
JournalScience
Volume293
Issue number5529
DOIs
StatePublished - Jul 20 2001
Externally publishedYes

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Streptococcus pneumoniae
Genome
Membrane Proteins
Hexosamines
Comparative Genomic Hybridization
Insertional Mutagenesis
Cytosine
Guanine
Otitis Media
Protein Sorting Signals
Bacteremia
Oligonucleotide Array Sequence Analysis
Meningitis
Base Pairing
Polysaccharides
Virulence
Pneumonia
Nitrogen
Carbon
Vaccines

ASJC Scopus subject areas

  • General

Cite this

Tettelin, H., Nelson, K. E., Paulsen, I. T., Eisen, J. A., Read, T. D., Peterson, S., ... Fraser, C. M. (2001). Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science, 293(5529), 498-506. https://doi.org/10.1126/science.1061217

Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. / Tettelin, H.; Nelson, K. E.; Paulsen, I. T.; Eisen, Jonathan A; Read, T. D.; Peterson, S.; Heidelberg, J.; DeBoy, R. T.; Haft, D. H.; Dodson, R. J.; Durkin, A. S.; Gwinn, M.; Kolonay, J. F.; Nelson, W. C.; Peterson, J. D.; Umayam, L. A.; White, O.; Salzberg, S. L.; Lewis, M. R.; Radune, D.; Holtzapple, E.; Khouri, H.; Wolf, A. M.; Utterback, T. R.; Hansen, C. L.; McDonald, L. A.; Feldblyum, T. V.; Angiuoli, S.; Dickinson, T.; Hickey, E. K.; Holt, I. E.; Loftus, B. J.; Yang, F.; Smith, H. O.; Venter, J. C.; Dougherty, B. A.; Morrison, D. A.; Hollingshead, S. K.; Fraser, C. M.

In: Science, Vol. 293, No. 5529, 20.07.2001, p. 498-506.

Research output: Contribution to journalArticle

Tettelin, H, Nelson, KE, Paulsen, IT, Eisen, JA, Read, TD, Peterson, S, Heidelberg, J, DeBoy, RT, Haft, DH, Dodson, RJ, Durkin, AS, Gwinn, M, Kolonay, JF, Nelson, WC, Peterson, JD, Umayam, LA, White, O, Salzberg, SL, Lewis, MR, Radune, D, Holtzapple, E, Khouri, H, Wolf, AM, Utterback, TR, Hansen, CL, McDonald, LA, Feldblyum, TV, Angiuoli, S, Dickinson, T, Hickey, EK, Holt, IE, Loftus, BJ, Yang, F, Smith, HO, Venter, JC, Dougherty, BA, Morrison, DA, Hollingshead, SK & Fraser, CM 2001, 'Complete genome sequence of a virulent isolate of Streptococcus pneumoniae', Science, vol. 293, no. 5529, pp. 498-506. https://doi.org/10.1126/science.1061217
Tettelin H, Nelson KE, Paulsen IT, Eisen JA, Read TD, Peterson S et al. Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science. 2001 Jul 20;293(5529):498-506. https://doi.org/10.1126/science.1061217
Tettelin, H. ; Nelson, K. E. ; Paulsen, I. T. ; Eisen, Jonathan A ; Read, T. D. ; Peterson, S. ; Heidelberg, J. ; DeBoy, R. T. ; Haft, D. H. ; Dodson, R. J. ; Durkin, A. S. ; Gwinn, M. ; Kolonay, J. F. ; Nelson, W. C. ; Peterson, J. D. ; Umayam, L. A. ; White, O. ; Salzberg, S. L. ; Lewis, M. R. ; Radune, D. ; Holtzapple, E. ; Khouri, H. ; Wolf, A. M. ; Utterback, T. R. ; Hansen, C. L. ; McDonald, L. A. ; Feldblyum, T. V. ; Angiuoli, S. ; Dickinson, T. ; Hickey, E. K. ; Holt, I. E. ; Loftus, B. J. ; Yang, F. ; Smith, H. O. ; Venter, J. C. ; Dougherty, B. A. ; Morrison, D. A. ; Hollingshead, S. K. ; Fraser, C. M. / Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. In: Science. 2001 ; Vol. 293, No. 5529. pp. 498-506.
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T1 - Complete genome sequence of a virulent isolate of Streptococcus pneumoniae

AU - Tettelin, H.

AU - Nelson, K. E.

AU - Paulsen, I. T.

AU - Eisen, Jonathan A

AU - Read, T. D.

AU - Peterson, S.

AU - Heidelberg, J.

AU - DeBoy, R. T.

AU - Haft, D. H.

AU - Dodson, R. J.

AU - Durkin, A. S.

AU - Gwinn, M.

AU - Kolonay, J. F.

AU - Nelson, W. C.

AU - Peterson, J. D.

AU - Umayam, L. A.

AU - White, O.

AU - Salzberg, S. L.

AU - Lewis, M. R.

AU - Radune, D.

AU - Holtzapple, E.

AU - Khouri, H.

AU - Wolf, A. M.

AU - Utterback, T. R.

AU - Hansen, C. L.

AU - McDonald, L. A.

AU - Feldblyum, T. V.

AU - Angiuoli, S.

AU - Dickinson, T.

AU - Hickey, E. K.

AU - Holt, I. E.

AU - Loftus, B. J.

AU - Yang, F.

AU - Smith, H. O.

AU - Venter, J. C.

AU - Dougherty, B. A.

AU - Morrison, D. A.

AU - Hollingshead, S. K.

AU - Fraser, C. M.

PY - 2001/7/20

Y1 - 2001/7/20

N2 - The 2,160,837-base pair genome sequence of an isolate of Streptococcus pneumoniae, a Gram-positive pathogen that causes pneumonia, bacteremia, meningitis, and otitis media, contains 2236 predicted coding regions; of these, 1440 (64%) were assigned a biological role. Approximately 5% of the genome is composed of insertion sequences that may contribute to genome rearrangements through uptake of foreign DNA. Extracellular enzyme systems for the metabolism of polysaccharides and hexosamines provide a substantial source of carbon and nitrogen for S. pneumoniae and also damage host tissues and facilitate colonization. A motif identified within the signal peptide of proteins is potentially involved in targeting these proteins to the cell surface of low-guanine/cytosine (GC) Gram-positive species. Several surface-exposed proteins that may serve as potential vaccine candidates were identified. Comparative genome hybridization with DNA arrays revealed strain differences in S. pneumoniae that could contribute to differences in virulence and antigenicity.

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