Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae

Hervé Tettelin, Vega Masignani, Michael J. Cieslewicz, Jonathan A Eisen, Scott Peterson, Michael R. Wessels, Ian T. Paulsen, Karen E. Nelson, Immaculada Margarit, Timothy D. Read, Lawrence C. Madoff, Alex M. Wolf, Maureen J. Beanan, Lauren M. Brinkac, Sean C. Daugherty, Robert T. DeBoy, A. Scott Durkin, James F. Kolonay, Ramana Madupu, Matthew R. LewisDiana Radune, Nadezhda B. Fedorova, David Scanlan, Hoda Khouri, Stephanie Mulligan, Heather A. Carty, Robin T. Cline, Susan E. Van Aken, John Gill, Maria Scarselli, Marirosa Mora, Emilia T. Iacobini, Cecilia Brettoni, Giuliano Galli, Massimo Mariani, Filippo Vegni, Domenico Maione, Daniela Rinaudo, Rino Rappuoli, John L. Telford, Dennis L. Kasper, Guido Grandi, Claire M. Fraser

Research output: Contribution to journalArticle

356 Scopus citations

Abstract

The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.

Original languageEnglish (US)
Pages (from-to)12391-12396
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number19
DOIs
StatePublished - Sep 17 2002
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

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    Tettelin, H., Masignani, V., Cieslewicz, M. J., Eisen, J. A., Peterson, S., Wessels, M. R., Paulsen, I. T., Nelson, K. E., Margarit, I., Read, T. D., Madoff, L. C., Wolf, A. M., Beanan, M. J., Brinkac, L. M., Daugherty, S. C., DeBoy, R. T., Durkin, A. S., Kolonay, J. F., Madupu, R., ... Fraser, C. M. (2002). Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proceedings of the National Academy of Sciences of the United States of America, 99(19), 12391-12396. https://doi.org/10.1073/pnas.182380799