Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae

Hervé Tettelin, Vega Masignani, Michael J. Cieslewicz, Jonathan A Eisen, Scott Peterson, Michael R. Wessels, Ian T. Paulsen, Karen E. Nelson, Immaculada Margarit, Timothy D. Read, Lawrence C. Madoff, Alex M. Wolf, Maureen J. Beanan, Lauren M. Brinkac, Sean C. Daugherty, Robert T. DeBoy, A. Scott Durkin, James F. Kolonay, Ramana Madupu, Matthew R. LewisDiana Radune, Nadezhda B. Fedorova, David Scanlan, Hoda Khouri, Stephanie Mulligan, Heather A. Carty, Robin T. Cline, Susan E. Van Aken, John Gill, Maria Scarselli, Marirosa Mora, Emilia T. Iacobini, Cecilia Brettoni, Giuliano Galli, Massimo Mariani, Filippo Vegni, Domenico Maione, Daniela Rinaudo, Rino Rappuoli, John L. Telford, Dennis L. Kasper, Guido Grandi, Claire M. Fraser

Research output: Contribution to journalArticle

354 Citations (Scopus)

Abstract

The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.

Original languageEnglish (US)
Pages (from-to)12391-12396
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number19
DOIs
StatePublished - Sep 17 2002
Externally publishedYes

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Streptococcus agalactiae
Genome
Bacterial Pneumonia
Bacterial Meningitides
Comparative Genomic Hybridization
Genetic Heterogeneity
Streptococcus pyogenes
Streptococcus pneumoniae
Streptococcus
Computer Simulation
Genes
Virulence
Sepsis
Serogroup

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. / Tettelin, Hervé; Masignani, Vega; Cieslewicz, Michael J.; Eisen, Jonathan A; Peterson, Scott; Wessels, Michael R.; Paulsen, Ian T.; Nelson, Karen E.; Margarit, Immaculada; Read, Timothy D.; Madoff, Lawrence C.; Wolf, Alex M.; Beanan, Maureen J.; Brinkac, Lauren M.; Daugherty, Sean C.; DeBoy, Robert T.; Durkin, A. Scott; Kolonay, James F.; Madupu, Ramana; Lewis, Matthew R.; Radune, Diana; Fedorova, Nadezhda B.; Scanlan, David; Khouri, Hoda; Mulligan, Stephanie; Carty, Heather A.; Cline, Robin T.; Van Aken, Susan E.; Gill, John; Scarselli, Maria; Mora, Marirosa; Iacobini, Emilia T.; Brettoni, Cecilia; Galli, Giuliano; Mariani, Massimo; Vegni, Filippo; Maione, Domenico; Rinaudo, Daniela; Rappuoli, Rino; Telford, John L.; Kasper, Dennis L.; Grandi, Guido; Fraser, Claire M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 19, 17.09.2002, p. 12391-12396.

Research output: Contribution to journalArticle

Tettelin, H, Masignani, V, Cieslewicz, MJ, Eisen, JA, Peterson, S, Wessels, MR, Paulsen, IT, Nelson, KE, Margarit, I, Read, TD, Madoff, LC, Wolf, AM, Beanan, MJ, Brinkac, LM, Daugherty, SC, DeBoy, RT, Durkin, AS, Kolonay, JF, Madupu, R, Lewis, MR, Radune, D, Fedorova, NB, Scanlan, D, Khouri, H, Mulligan, S, Carty, HA, Cline, RT, Van Aken, SE, Gill, J, Scarselli, M, Mora, M, Iacobini, ET, Brettoni, C, Galli, G, Mariani, M, Vegni, F, Maione, D, Rinaudo, D, Rappuoli, R, Telford, JL, Kasper, DL, Grandi, G & Fraser, CM 2002, 'Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 19, pp. 12391-12396. https://doi.org/10.1073/pnas.182380799
Tettelin, Hervé ; Masignani, Vega ; Cieslewicz, Michael J. ; Eisen, Jonathan A ; Peterson, Scott ; Wessels, Michael R. ; Paulsen, Ian T. ; Nelson, Karen E. ; Margarit, Immaculada ; Read, Timothy D. ; Madoff, Lawrence C. ; Wolf, Alex M. ; Beanan, Maureen J. ; Brinkac, Lauren M. ; Daugherty, Sean C. ; DeBoy, Robert T. ; Durkin, A. Scott ; Kolonay, James F. ; Madupu, Ramana ; Lewis, Matthew R. ; Radune, Diana ; Fedorova, Nadezhda B. ; Scanlan, David ; Khouri, Hoda ; Mulligan, Stephanie ; Carty, Heather A. ; Cline, Robin T. ; Van Aken, Susan E. ; Gill, John ; Scarselli, Maria ; Mora, Marirosa ; Iacobini, Emilia T. ; Brettoni, Cecilia ; Galli, Giuliano ; Mariani, Massimo ; Vegni, Filippo ; Maione, Domenico ; Rinaudo, Daniela ; Rappuoli, Rino ; Telford, John L. ; Kasper, Dennis L. ; Grandi, Guido ; Fraser, Claire M. / Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 19. pp. 12391-12396.
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abstract = "The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.",
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AU - Masignani, Vega

AU - Cieslewicz, Michael J.

AU - Eisen, Jonathan A

AU - Peterson, Scott

AU - Wessels, Michael R.

AU - Paulsen, Ian T.

AU - Nelson, Karen E.

AU - Margarit, Immaculada

AU - Read, Timothy D.

AU - Madoff, Lawrence C.

AU - Wolf, Alex M.

AU - Beanan, Maureen J.

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AU - DeBoy, Robert T.

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AU - Kolonay, James F.

AU - Madupu, Ramana

AU - Lewis, Matthew R.

AU - Radune, Diana

AU - Fedorova, Nadezhda B.

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AU - Khouri, Hoda

AU - Mulligan, Stephanie

AU - Carty, Heather A.

AU - Cline, Robin T.

AU - Van Aken, Susan E.

AU - Gill, John

AU - Scarselli, Maria

AU - Mora, Marirosa

AU - Iacobini, Emilia T.

AU - Brettoni, Cecilia

AU - Galli, Giuliano

AU - Mariani, Massimo

AU - Vegni, Filippo

AU - Maione, Domenico

AU - Rinaudo, Daniela

AU - Rappuoli, Rino

AU - Telford, John L.

AU - Kasper, Dennis L.

AU - Grandi, Guido

AU - Fraser, Claire M.

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