Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

K. E. Nelson, C. Weinel, I. T. Paulsen, R. J. Dodson, H. Hilbert, V. A P Martins dos Santos, D. E. Fouts, S. R. Gill, M. Pop, M. Holmes, L. Brinkac, M. Beanan, R. T. DeBoy, S. Daugherty, J. Kolonay, R. Madupu, W. Nelson, O. White, J. Peterson, H. Khouri & 23 others I. Hance, P. Chris Lee, E. Holtzapple, D. Scanlan, K. Tran, A. Moazzez, T. Utterback, M. Rizzo, K. Lee, D. Kosack, D. Moestl, H. Wedler, J. Lauber, D. Stjepandic, J. Hoheisel, M. Straetz, S. Heim, C. Kiewitz, Jonathan A Eisen, K. N. Timmis, A. Düsterhöft, B. Tümmler, C. M. Fraser

Research output: Contribution to journalArticle

855 Citations (Scopus)

Abstract

Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.

Original languageEnglish (US)
Pages (from-to)799-808
Number of pages10
JournalEnvironmental Microbiology
Volume4
Issue number12
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Pseudomonas putida
Sequence Analysis
genome
Genome
Biocatalysis
biosafety
Bacteria
exotoxins
type III secretion system
Exotoxins
bacterium
Environmental Biodegradation
bioremediation
soil bacteria
Virulence Factors
virulence
Agriculture
Pseudomonas aeruginosa
secretion
Organism Cloning

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Nelson, K. E., Weinel, C., Paulsen, I. T., Dodson, R. J., Hilbert, H., Martins dos Santos, V. A. P., ... Fraser, C. M. (2002). Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental Microbiology, 4(12), 799-808. https://doi.org/10.1046/j.1462-2920.2002.00366.x

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. / Nelson, K. E.; Weinel, C.; Paulsen, I. T.; Dodson, R. J.; Hilbert, H.; Martins dos Santos, V. A P; Fouts, D. E.; Gill, S. R.; Pop, M.; Holmes, M.; Brinkac, L.; Beanan, M.; DeBoy, R. T.; Daugherty, S.; Kolonay, J.; Madupu, R.; Nelson, W.; White, O.; Peterson, J.; Khouri, H.; Hance, I.; Chris Lee, P.; Holtzapple, E.; Scanlan, D.; Tran, K.; Moazzez, A.; Utterback, T.; Rizzo, M.; Lee, K.; Kosack, D.; Moestl, D.; Wedler, H.; Lauber, J.; Stjepandic, D.; Hoheisel, J.; Straetz, M.; Heim, S.; Kiewitz, C.; Eisen, Jonathan A; Timmis, K. N.; Düsterhöft, A.; Tümmler, B.; Fraser, C. M.

In: Environmental Microbiology, Vol. 4, No. 12, 01.12.2002, p. 799-808.

Research output: Contribution to journalArticle

Nelson, KE, Weinel, C, Paulsen, IT, Dodson, RJ, Hilbert, H, Martins dos Santos, VAP, Fouts, DE, Gill, SR, Pop, M, Holmes, M, Brinkac, L, Beanan, M, DeBoy, RT, Daugherty, S, Kolonay, J, Madupu, R, Nelson, W, White, O, Peterson, J, Khouri, H, Hance, I, Chris Lee, P, Holtzapple, E, Scanlan, D, Tran, K, Moazzez, A, Utterback, T, Rizzo, M, Lee, K, Kosack, D, Moestl, D, Wedler, H, Lauber, J, Stjepandic, D, Hoheisel, J, Straetz, M, Heim, S, Kiewitz, C, Eisen, JA, Timmis, KN, Düsterhöft, A, Tümmler, B & Fraser, CM 2002, 'Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440', Environmental Microbiology, vol. 4, no. 12, pp. 799-808. https://doi.org/10.1046/j.1462-2920.2002.00366.x
Nelson, K. E. ; Weinel, C. ; Paulsen, I. T. ; Dodson, R. J. ; Hilbert, H. ; Martins dos Santos, V. A P ; Fouts, D. E. ; Gill, S. R. ; Pop, M. ; Holmes, M. ; Brinkac, L. ; Beanan, M. ; DeBoy, R. T. ; Daugherty, S. ; Kolonay, J. ; Madupu, R. ; Nelson, W. ; White, O. ; Peterson, J. ; Khouri, H. ; Hance, I. ; Chris Lee, P. ; Holtzapple, E. ; Scanlan, D. ; Tran, K. ; Moazzez, A. ; Utterback, T. ; Rizzo, M. ; Lee, K. ; Kosack, D. ; Moestl, D. ; Wedler, H. ; Lauber, J. ; Stjepandic, D. ; Hoheisel, J. ; Straetz, M. ; Heim, S. ; Kiewitz, C. ; Eisen, Jonathan A ; Timmis, K. N. ; Düsterhöft, A. ; Tümmler, B. ; Fraser, C. M. / Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. In: Environmental Microbiology. 2002 ; Vol. 4, No. 12. pp. 799-808.
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AU - Weinel, C.

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AU - Dodson, R. J.

AU - Hilbert, H.

AU - Martins dos Santos, V. A P

AU - Fouts, D. E.

AU - Gill, S. R.

AU - Pop, M.

AU - Holmes, M.

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AU - Beanan, M.

AU - DeBoy, R. T.

AU - Daugherty, S.

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AU - White, O.

AU - Peterson, J.

AU - Khouri, H.

AU - Hance, I.

AU - Chris Lee, P.

AU - Holtzapple, E.

AU - Scanlan, D.

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AU - Moazzez, A.

AU - Utterback, T.

AU - Rizzo, M.

AU - Lee, K.

AU - Kosack, D.

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AU - Wedler, H.

AU - Lauber, J.

AU - Stjepandic, D.

AU - Hoheisel, J.

AU - Straetz, M.

AU - Heim, S.

AU - Kiewitz, C.

AU - Eisen, Jonathan A

AU - Timmis, K. N.

AU - Düsterhöft, A.

AU - Tümmler, B.

AU - Fraser, C. M.

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