Complement activation by 19S IgM rheumatoid factor: Relationship to disease activity in rheumatoid arthritis

D. L. Robbins, D. W. Fiegal, J. C. Leek, R. Shapiro, K. Wiesner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

19S IgM rheumatoid factor (RF) in rheumatoid arthritis (RA) are polyclonal auto-antibodies directed against the Fc piece of IgG. Rheumatoid patients with RF tend to have aggressive synovitis, nodules, and extraarticular manifestations. Alhough RF titer does not correlate with disease activity, RF activates complement (C) by the classical pathway. Thus, we postulated that selective stimulation of cell clones producing efficient C activating RF molecules might be associated with disease flares, independent of changes in serum RF concentration. To address the question, 42 patients with RA were evaluated prospectively. Serum RF cncentration was measured by radioimmunoassay (RIA) and C activating activity by hemolytic assay. We then calculated the mean hemolysis (MH) of sensitized sheep erythrocytes (SRC) produced/ml of RF serum (MH/ml) and MH/μg of RF as an expression of RF C activating properties (CAP). The following observations were made: (1) RF CAP varied among the patients studied; (2) RF CAP varied over time in individual patients; (3) RF CAP differences varied in both groups independently from RF concentration; (4) RF CAP correlated with both systemic and articular disease activity; and (5) total RF concentration correlated with articular findings and nodules but less well with systemic disease activity.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalJournal of Rheumatology
Volume13
Issue number1
StatePublished - 1986

Fingerprint

Rheumatoid Factor
Complement Activation
Immunoglobulin M
Rheumatoid Arthritis
Hemolysis
Joints
Serum
Classical Complement Pathway
Synovitis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Robbins, D. L., Fiegal, D. W., Leek, J. C., Shapiro, R., & Wiesner, K. (1986). Complement activation by 19S IgM rheumatoid factor: Relationship to disease activity in rheumatoid arthritis. Journal of Rheumatology, 13(1), 33-38.

Complement activation by 19S IgM rheumatoid factor : Relationship to disease activity in rheumatoid arthritis. / Robbins, D. L.; Fiegal, D. W.; Leek, J. C.; Shapiro, R.; Wiesner, K.

In: Journal of Rheumatology, Vol. 13, No. 1, 1986, p. 33-38.

Research output: Contribution to journalArticle

Robbins, DL, Fiegal, DW, Leek, JC, Shapiro, R & Wiesner, K 1986, 'Complement activation by 19S IgM rheumatoid factor: Relationship to disease activity in rheumatoid arthritis', Journal of Rheumatology, vol. 13, no. 1, pp. 33-38.
Robbins, D. L. ; Fiegal, D. W. ; Leek, J. C. ; Shapiro, R. ; Wiesner, K. / Complement activation by 19S IgM rheumatoid factor : Relationship to disease activity in rheumatoid arthritis. In: Journal of Rheumatology. 1986 ; Vol. 13, No. 1. pp. 33-38.
@article{32fa7adf734442219ddc418afb504b2d,
title = "Complement activation by 19S IgM rheumatoid factor: Relationship to disease activity in rheumatoid arthritis",
abstract = "19S IgM rheumatoid factor (RF) in rheumatoid arthritis (RA) are polyclonal auto-antibodies directed against the Fc piece of IgG. Rheumatoid patients with RF tend to have aggressive synovitis, nodules, and extraarticular manifestations. Alhough RF titer does not correlate with disease activity, RF activates complement (C) by the classical pathway. Thus, we postulated that selective stimulation of cell clones producing efficient C activating RF molecules might be associated with disease flares, independent of changes in serum RF concentration. To address the question, 42 patients with RA were evaluated prospectively. Serum RF cncentration was measured by radioimmunoassay (RIA) and C activating activity by hemolytic assay. We then calculated the mean hemolysis (MH) of sensitized sheep erythrocytes (SRC) produced/ml of RF serum (MH/ml) and MH/μg of RF as an expression of RF C activating properties (CAP). The following observations were made: (1) RF CAP varied among the patients studied; (2) RF CAP varied over time in individual patients; (3) RF CAP differences varied in both groups independently from RF concentration; (4) RF CAP correlated with both systemic and articular disease activity; and (5) total RF concentration correlated with articular findings and nodules but less well with systemic disease activity.",
author = "Robbins, {D. L.} and Fiegal, {D. W.} and Leek, {J. C.} and R. Shapiro and K. Wiesner",
year = "1986",
language = "English (US)",
volume = "13",
pages = "33--38",
journal = "Journal of Rheumatology",
issn = "0315-162X",
publisher = "Journal of Rheumatology",
number = "1",

}

TY - JOUR

T1 - Complement activation by 19S IgM rheumatoid factor

T2 - Relationship to disease activity in rheumatoid arthritis

AU - Robbins, D. L.

AU - Fiegal, D. W.

AU - Leek, J. C.

AU - Shapiro, R.

AU - Wiesner, K.

PY - 1986

Y1 - 1986

N2 - 19S IgM rheumatoid factor (RF) in rheumatoid arthritis (RA) are polyclonal auto-antibodies directed against the Fc piece of IgG. Rheumatoid patients with RF tend to have aggressive synovitis, nodules, and extraarticular manifestations. Alhough RF titer does not correlate with disease activity, RF activates complement (C) by the classical pathway. Thus, we postulated that selective stimulation of cell clones producing efficient C activating RF molecules might be associated with disease flares, independent of changes in serum RF concentration. To address the question, 42 patients with RA were evaluated prospectively. Serum RF cncentration was measured by radioimmunoassay (RIA) and C activating activity by hemolytic assay. We then calculated the mean hemolysis (MH) of sensitized sheep erythrocytes (SRC) produced/ml of RF serum (MH/ml) and MH/μg of RF as an expression of RF C activating properties (CAP). The following observations were made: (1) RF CAP varied among the patients studied; (2) RF CAP varied over time in individual patients; (3) RF CAP differences varied in both groups independently from RF concentration; (4) RF CAP correlated with both systemic and articular disease activity; and (5) total RF concentration correlated with articular findings and nodules but less well with systemic disease activity.

AB - 19S IgM rheumatoid factor (RF) in rheumatoid arthritis (RA) are polyclonal auto-antibodies directed against the Fc piece of IgG. Rheumatoid patients with RF tend to have aggressive synovitis, nodules, and extraarticular manifestations. Alhough RF titer does not correlate with disease activity, RF activates complement (C) by the classical pathway. Thus, we postulated that selective stimulation of cell clones producing efficient C activating RF molecules might be associated with disease flares, independent of changes in serum RF concentration. To address the question, 42 patients with RA were evaluated prospectively. Serum RF cncentration was measured by radioimmunoassay (RIA) and C activating activity by hemolytic assay. We then calculated the mean hemolysis (MH) of sensitized sheep erythrocytes (SRC) produced/ml of RF serum (MH/ml) and MH/μg of RF as an expression of RF C activating properties (CAP). The following observations were made: (1) RF CAP varied among the patients studied; (2) RF CAP varied over time in individual patients; (3) RF CAP differences varied in both groups independently from RF concentration; (4) RF CAP correlated with both systemic and articular disease activity; and (5) total RF concentration correlated with articular findings and nodules but less well with systemic disease activity.

UR - http://www.scopus.com/inward/record.url?scp=0022611316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022611316&partnerID=8YFLogxK

M3 - Article

C2 - 3701741

AN - SCOPUS:0022611316

VL - 13

SP - 33

EP - 38

JO - Journal of Rheumatology

JF - Journal of Rheumatology

SN - 0315-162X

IS - 1

ER -