Competitive immunochromatographic assay for the detection of the organophosphorus pesticide EPN

Eun Hye Lee, Young Ah Kim, Yong Tae Lee, Bruce D. Hammock, Hye Sung Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An immunochromatographic assay (ICA) based on competitive antigen-coated format and colloidal gold label was developed for the detection of the organophosphorus insecticide O-ethyl O-4-nitrophenyl phenylphosphonothioate (EPN). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with EPN hapten-OVA conjugate (test line) and anti-mouse IgG (control line). As the competition is between the migrating analyte and the immobilised analyte hapten for the binding sites of the migrating antibody-colloidal gold (Ab-CG) conjugate, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. Based on this criterion, a nitrocellulose (NC) membrane was chosen as the most suitable for EPN ICA. The detection limit of the ICA for EPN standard and EPN spiked into agricultural samples were 10-2 and 5×10-2 μg mL-1, respectively.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalFood and Agricultural Immunology
Volume24
Issue number2
DOIs
StatePublished - Jun 2013

Fingerprint

Immunochromatography
immunoaffinity chromatography
organophosphorus pesticides
Pesticides
Gold Colloid
haptens
Haptens
2-Ethyl 2-(4-Nitrophenyl) Ester Phenylphosphonothioic Acid
gold
Membranes
Absorbent Pads
Antibody Binding Sites
absorbents
Collodion
organophosphorus insecticides
Insecticides
Limit of Detection
detection limit
testing
antigens

Keywords

  • EPN
  • immunoassay
  • immunochromatographic assay
  • organophosphorus pesticide

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Agronomy and Crop Science

Cite this

Competitive immunochromatographic assay for the detection of the organophosphorus pesticide EPN. / Lee, Eun Hye; Kim, Young Ah; Lee, Yong Tae; Hammock, Bruce D.; Lee, Hye Sung.

In: Food and Agricultural Immunology, Vol. 24, No. 2, 06.2013, p. 129-138.

Research output: Contribution to journalArticle

Lee, Eun Hye ; Kim, Young Ah ; Lee, Yong Tae ; Hammock, Bruce D. ; Lee, Hye Sung. / Competitive immunochromatographic assay for the detection of the organophosphorus pesticide EPN. In: Food and Agricultural Immunology. 2013 ; Vol. 24, No. 2. pp. 129-138.
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