Abstract
An immunochromatographic assay (ICA) based on competitive antigen-coated format and colloidal gold label was developed for the detection of the organophosphorus insecticide O-ethyl O-4-nitrophenyl phenylphosphonothioate (EPN). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with EPN hapten-OVA conjugate (test line) and anti-mouse IgG (control line). As the competition is between the migrating analyte and the immobilised analyte hapten for the binding sites of the migrating antibody-colloidal gold (Ab-CG) conjugate, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. Based on this criterion, a nitrocellulose (NC) membrane was chosen as the most suitable for EPN ICA. The detection limit of the ICA for EPN standard and EPN spiked into agricultural samples were 10-2 and 5×10-2 μg mL-1, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 129-138 |
| Number of pages | 10 |
| Journal | Food and Agricultural Immunology |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 2013 |
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Keywords
- EPN
- immunoassay
- immunochromatographic assay
- organophosphorus pesticide
ASJC Scopus subject areas
- Food Science
- Immunology
- Agronomy and Crop Science
Cite this
Competitive immunochromatographic assay for the detection of the organophosphorus pesticide EPN. / Lee, Eun Hye; Kim, Young Ah; Lee, Yong Tae; Hammock, Bruce D.; Lee, Hye Sung.
In: Food and Agricultural Immunology, Vol. 24, No. 2, 06.2013, p. 129-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Competitive immunochromatographic assay for the detection of the organophosphorus pesticide EPN
AU - Lee, Eun Hye
AU - Kim, Young Ah
AU - Lee, Yong Tae
AU - Hammock, Bruce D.
AU - Lee, Hye Sung
PY - 2013/6
Y1 - 2013/6
N2 - An immunochromatographic assay (ICA) based on competitive antigen-coated format and colloidal gold label was developed for the detection of the organophosphorus insecticide O-ethyl O-4-nitrophenyl phenylphosphonothioate (EPN). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with EPN hapten-OVA conjugate (test line) and anti-mouse IgG (control line). As the competition is between the migrating analyte and the immobilised analyte hapten for the binding sites of the migrating antibody-colloidal gold (Ab-CG) conjugate, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. Based on this criterion, a nitrocellulose (NC) membrane was chosen as the most suitable for EPN ICA. The detection limit of the ICA for EPN standard and EPN spiked into agricultural samples were 10-2 and 5×10-2 μg mL-1, respectively.
AB - An immunochromatographic assay (ICA) based on competitive antigen-coated format and colloidal gold label was developed for the detection of the organophosphorus insecticide O-ethyl O-4-nitrophenyl phenylphosphonothioate (EPN). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with EPN hapten-OVA conjugate (test line) and anti-mouse IgG (control line). As the competition is between the migrating analyte and the immobilised analyte hapten for the binding sites of the migrating antibody-colloidal gold (Ab-CG) conjugate, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. Based on this criterion, a nitrocellulose (NC) membrane was chosen as the most suitable for EPN ICA. The detection limit of the ICA for EPN standard and EPN spiked into agricultural samples were 10-2 and 5×10-2 μg mL-1, respectively.
KW - EPN
KW - immunoassay
KW - immunochromatographic assay
KW - organophosphorus pesticide
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U2 - 10.1080/09540105.2012.661703
DO - 10.1080/09540105.2012.661703
M3 - Article
VL - 24
SP - 129
EP - 138
JO - Food and Agricultural Immunology
JF - Food and Agricultural Immunology
SN - 0954-0105
IS - 2
ER -