Abstract
An immunochromatographic assay (ICA) based on competitive antigen-coated format and colloidal gold label was developed for the detection of the organophosphorus insecticide O-ethyl O-4-nitrophenyl phenylphosphonothioate (EPN). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with EPN hapten-OVA conjugate (test line) and anti-mouse IgG (control line). As the competition is between the migrating analyte and the immobilised analyte hapten for the binding sites of the migrating antibody-colloidal gold (Ab-CG) conjugate, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. Based on this criterion, a nitrocellulose (NC) membrane was chosen as the most suitable for EPN ICA. The detection limit of the ICA for EPN standard and EPN spiked into agricultural samples were 10-2 and 5×10-2 μg mL-1, respectively.
Original language | English (US) |
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Pages (from-to) | 129-138 |
Number of pages | 10 |
Journal | Food and Agricultural Immunology |
Volume | 24 |
Issue number | 2 |
DOIs | |
State | Published - Jun 2013 |
Keywords
- EPN
- immunoassay
- immunochromatographic assay
- organophosphorus pesticide
ASJC Scopus subject areas
- Food Science
- Immunology
- Agronomy and Crop Science