Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin

Xiude Hua, Liangliang Zhou, Lu Feng, Yuan Ding, Haiyan Shi, Limin Wang, Shirley J. Gee, Bruce D. Hammock, Minghua Wang

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Twenty-three phage-displayed peptides that specifically bind to an anti-benzothiostrobin monoclonal antibody (mAb) in the absence or presence of benzothiostrobin were isolated from a cyclic 8-residue peptide phage library. Competitive and noncompetitive phage enzyme linked immunosorbent assays (ELISAs) for benzothiostrobin were developed by using a clone C3-3 specific to the benzothiostrobin-free mAb and a clone N6-18 specific to the benzothiostrobin immunocomplex, respectively. Under the optimal conditions, the half maximal inhibition concentration (IC<inf>50</inf>) of the competitive phage ELISA and the concentration of analyte producing 50% saturation of the signal (SC<inf>50</inf>) of the noncompetitive phage ELISA for benzothiostrobin were 0.94 and 2.27 ng mL<sup>-1</sup>, respectively. The noncompetitive phage ELISA showed higher selectivity compared to the competitive. Recoveries of the competitive and the noncompetitive phage ELISAs for benzothiostrobin in cucumber, tomato, pear and rice samples were 67.6-119.6% and 70.4-125.0%, respectively. The amounts of benzothiostrobin in the containing incurred residues samples detected by the two types of phage ELISAs were significantly correlated with that detected by high-performance liquid chromatography (HPLC).

Original languageEnglish (US)
Article number234081
Pages (from-to)150-156
Number of pages7
JournalAnalytica Chimica Acta
Volume890
DOIs
StatePublished - Aug 26 2015

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Keywords

  • Benzothiostrobin
  • Enzyme-linked immunosorbent assay
  • Noncompetitive immunoassay
  • Peptidomimetics
  • Phage anti-immunocomplex assay
  • Phage-displayed peptide

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Hua, X., Zhou, L., Feng, L., Ding, Y., Shi, H., Wang, L., Gee, S. J., Hammock, B. D., & Wang, M. (2015). Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin. Analytica Chimica Acta, 890, 150-156. [234081]. https://doi.org/10.1016/j.aca.2015.07.056