Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization

Alexis J. Lomakin, Kun Chun Lee, Sangyoon J. Han, Duyen A. Bui, Michael Davidson, Alex Mogilner, Gaudenz Danuser

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Symmetry-breaking polarization enables functional plasticity of cells and tissues and is yet not well understood. Here we show that epithelial cells, hard-wired to maintain a static morphology and to preserve tissue organization, can spontaneously switch to a migratory polarized phenotype after relaxation of the actomyosin cytoskeleton. We find that myosin II engages actin in the formation of cortical actomyosin bundles and thus makes it unavailable for deployment in the process of dendritic growth normally driving cell motility. Under low-contractility regimes, epithelial cells polarize in a front-back manner owing to the emergence of actin retrograde flows powered by dendritic polymerization of actin. Coupled to cell movement, the flows transport myosin II from the front to the back of the cell, where the motor locally 'locks' actin in contractile bundles. This polarization mechanism could be employed by embryonic and cancer epithelial cells in microenvironments where high-contractility-driven cell motion is inefficient.

Original languageEnglish (US)
Pages (from-to)1435-1445
Number of pages11
JournalNature Cell Biology
Volume17
Issue number11
DOIs
StatePublished - Nov 1 2015

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Actins
Myosin Type II
Actomyosin
Epithelial Cells
Cell Movement
Cellular Microenvironment
Cytoskeleton
Polymerization
Phenotype
Growth
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lomakin, A. J., Lee, K. C., Han, S. J., Bui, D. A., Davidson, M., Mogilner, A., & Danuser, G. (2015). Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization. Nature Cell Biology, 17(11), 1435-1445. https://doi.org/10.1038/ncb3246

Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization. / Lomakin, Alexis J.; Lee, Kun Chun; Han, Sangyoon J.; Bui, Duyen A.; Davidson, Michael; Mogilner, Alex; Danuser, Gaudenz.

In: Nature Cell Biology, Vol. 17, No. 11, 01.11.2015, p. 1435-1445.

Research output: Contribution to journalArticle

Lomakin, AJ, Lee, KC, Han, SJ, Bui, DA, Davidson, M, Mogilner, A & Danuser, G 2015, 'Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization', Nature Cell Biology, vol. 17, no. 11, pp. 1435-1445. https://doi.org/10.1038/ncb3246
Lomakin, Alexis J. ; Lee, Kun Chun ; Han, Sangyoon J. ; Bui, Duyen A. ; Davidson, Michael ; Mogilner, Alex ; Danuser, Gaudenz. / Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization. In: Nature Cell Biology. 2015 ; Vol. 17, No. 11. pp. 1435-1445.
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