Abstract
IS50R is a transposable genetic element that serves as the right inverted repeat of the transposon Tn5. Earlier work has shown that IS50R encodes at least two proteins (called P1 and P2) involved in transposition. In this paper, we described the localization properties of the proteins encoded on this repeat. Strains were constructed that over produced either these two proteins or hybrids between β-galactosidase and the IS50R proteins. An antiserum was raised against the hybrid proteins, and this was used to study the localization of P1 and P2. Based on studies in maxicells as well as in growing cells, we show that P1 and P2 are localized differently in the cell. P2 is a cytoplasmic protein, while P1 largely fractionates with the membrane.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3645-3651 |
| Number of pages | 7 |
| Journal | Journal of Biological Chemistry |
| Volume | 260 |
| Issue number | 6 |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Compartmentalization of the proteins encoded by IS50R. / Isberg, R. R.; Syvanen, Michael.
In: Journal of Biological Chemistry, Vol. 260, No. 6, 1985, p. 3645-3651.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Compartmentalization of the proteins encoded by IS50R
AU - Isberg, R. R.
AU - Syvanen, Michael
PY - 1985
Y1 - 1985
N2 - IS50R is a transposable genetic element that serves as the right inverted repeat of the transposon Tn5. Earlier work has shown that IS50R encodes at least two proteins (called P1 and P2) involved in transposition. In this paper, we described the localization properties of the proteins encoded on this repeat. Strains were constructed that over produced either these two proteins or hybrids between β-galactosidase and the IS50R proteins. An antiserum was raised against the hybrid proteins, and this was used to study the localization of P1 and P2. Based on studies in maxicells as well as in growing cells, we show that P1 and P2 are localized differently in the cell. P2 is a cytoplasmic protein, while P1 largely fractionates with the membrane.
AB - IS50R is a transposable genetic element that serves as the right inverted repeat of the transposon Tn5. Earlier work has shown that IS50R encodes at least two proteins (called P1 and P2) involved in transposition. In this paper, we described the localization properties of the proteins encoded on this repeat. Strains were constructed that over produced either these two proteins or hybrids between β-galactosidase and the IS50R proteins. An antiserum was raised against the hybrid proteins, and this was used to study the localization of P1 and P2. Based on studies in maxicells as well as in growing cells, we show that P1 and P2 are localized differently in the cell. P2 is a cytoplasmic protein, while P1 largely fractionates with the membrane.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021970014&partnerID=8YFLogxK
M3 - Article
C2 - 2982872
AN - SCOPUS:0021970014
VL - 260
SP - 3645
EP - 3651
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 6
ER -