Compartment-resolved imaging of cortical functional hyperemia with OCT angiography

Harsha Radhakrishnan, Vivek Srinivasan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Optical Coherence Tomography (OCT) angiography was applied to image functional hyperemia in different vascular compartments in the rat somatosensory cortex. Dynamic backscattering changes, indicative of changes in dynamic red blood cell (dRBC) content, were used to monitor the hemodynamic response. Three-dimensional movies depicting the microvascular response to neuronal activation were created for the first time. An increase in the attenuation coefficient during activation was identified, and a simple normalization procedure was proposed to correct for it. This procedure was applied to determine compartment-resolved backscattering changes caused by dRBC content changes during functional activation. Increases in dRBC content were observed in all vascular compartments (arterial, arteriolar, capillary, and venular), with the largest responses found in the arterial and arteriolar compartments. dRBC content increased with dilation in arteries but with barely detectable dilation in veins. dRBC content increased in capillaries without significant "all or none" capillary recruitment.

Original languageEnglish (US)
Pages (from-to)1255-1268
Number of pages14
JournalBiomedical Optics Express
Volume4
Issue number8
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

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angiography
Hyperemia
Optical Coherence Tomography
compartments
erythrocytes
Angiography
tomography
Erythrocytes
activation
Blood Vessels
Dilatation
backscattering
hemodynamic responses
Somatosensory Cortex
Motion Pictures
cortexes
attenuation coefficients
arteries
Veins
veins

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Compartment-resolved imaging of cortical functional hyperemia with OCT angiography. / Radhakrishnan, Harsha; Srinivasan, Vivek.

In: Biomedical Optics Express, Vol. 4, No. 8, 01.01.2013, p. 1255-1268.

Research output: Contribution to journalArticle

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