Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swab sampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies

R. L. Walker, Hailu Kinde, R. J. Anderson, A. E. Brown

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's recommended sampling method or a Moore swab sampling method demonstrated that the Moore swab method detected a greater number of positive samples (83.0% vs. 67.92%). When results using a conventional culture technique were compared to the VIDAS SLM assay using Moore swab sampling, there was good agreement (97.5%). When the VIDAS SLM assay was used to test in-line milk filters and compared to results from the conventional culture method, there was also good correlation between test results (95.57%). Overall, the VIDAS SLM assay using a modified sampling method compared favorably to the conventional culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negative samples.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalInternational Journal of Food Microbiology
Volume67
Issue number1-2
DOIs
StatePublished - Jul 20 2001

Keywords

  • Bulk tank milk
  • Detection methods
  • Milk filters
  • Salmonella
  • Unpasteurized milk

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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