Abstract
A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's recommended sampling method or a Moore swab sampling method demonstrated that the Moore swab method detected a greater number of positive samples (83.0% vs. 67.92%). When results using a conventional culture technique were compared to the VIDAS SLM assay using Moore swab sampling, there was good agreement (97.5%). When the VIDAS SLM assay was used to test in-line milk filters and compared to results from the conventional culture method, there was also good correlation between test results (95.57%). Overall, the VIDAS SLM assay using a modified sampling method compared favorably to the conventional culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negative samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 123-129 |
| Number of pages | 7 |
| Journal | International Journal of Food Microbiology |
| Volume | 67 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jul 20 2001 |
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Keywords
- Bulk tank milk
- Detection methods
- Milk filters
- Salmonella
- Unpasteurized milk
ASJC Scopus subject areas
- Food Science
- Microbiology
Cite this
Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swab sampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies. / Walker, R. L.; Kinde, Hailu; Anderson, R. J.; Brown, A. E.
In: International Journal of Food Microbiology, Vol. 67, No. 1-2, 20.07.2001, p. 123-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of VIDAS enzyme-linked fluorescent immunoassay using Moore swab sampling and conventional culture method for Salmonella detection in bulk tank milk and in-line milk filters in California dairies
AU - Walker, R. L.
AU - Kinde, Hailu
AU - Anderson, R. J.
AU - Brown, A. E.
PY - 2001/7/20
Y1 - 2001/7/20
N2 - A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's recommended sampling method or a Moore swab sampling method demonstrated that the Moore swab method detected a greater number of positive samples (83.0% vs. 67.92%). When results using a conventional culture technique were compared to the VIDAS SLM assay using Moore swab sampling, there was good agreement (97.5%). When the VIDAS SLM assay was used to test in-line milk filters and compared to results from the conventional culture method, there was also good correlation between test results (95.57%). Overall, the VIDAS SLM assay using a modified sampling method compared favorably to the conventional culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negative samples.
AB - A comparison of the VIDAS Salmonella (SLM) assay using the manufacturer's recommended sampling method or a Moore swab sampling method demonstrated that the Moore swab method detected a greater number of positive samples (83.0% vs. 67.92%). When results using a conventional culture technique were compared to the VIDAS SLM assay using Moore swab sampling, there was good agreement (97.5%). When the VIDAS SLM assay was used to test in-line milk filters and compared to results from the conventional culture method, there was also good correlation between test results (95.57%). Overall, the VIDAS SLM assay using a modified sampling method compared favorably to the conventional culture method and had the advantages of taking less time to obtain a negative or presumptive positive result, being less technically complicated and requiring less screening of non-lactose fermenting colonies from negative samples.
KW - Bulk tank milk
KW - Detection methods
KW - Milk filters
KW - Salmonella
KW - Unpasteurized milk
UR - http://www.scopus.com/inward/record.url?scp=0035919738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035919738&partnerID=8YFLogxK
U2 - 10.1016/S0168-1605(01)00427-5
DO - 10.1016/S0168-1605(01)00427-5
M3 - Article
C2 - 11482560
AN - SCOPUS:0035919738
VL - 67
SP - 123
EP - 129
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 1-2
ER -