Comparison of two tourniquet application times for regional intravenous limb perfusions with amikacin in sedated or anesthetized horses

F. A. Aristizabal, Jorge Nieto, A. G. Guedes, Julie E Dechant, S. Yamout, B. Morales, J. Snyder

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Regional limb perfusion (RLP) in horses has proven to be a simple and effective technique for the treatment of synovial and musculoskeletal infections in the distal portion of the limbs. The ideal tourniquet time needed to achieve therapeutic synovial concentrations remains unknown. The pharmacokinetic effects of general anesthesia (GA) versus standing sedation (SS) RLP on synovial amikacin concentrations are not completely understood. This study investigated the pharmacokinetic effects of RLP under general anesthesia (GA) versus standing sedation (SS) on synovial amikacin concentration following 20 or 30 min tourniquet time. Using 1 g of amikacin RLP was performed in two groups of six horses (GA and SS). A pneumatic tourniquet was applied proximal to the carpus and maintained for 20 or 30 min. Two weeks later, the opposite treatment (20 or 30 min) was randomly performed in the opposite limb of horses in each group (GA and SS).Synovial fluid samples were collected from the metacarpophalangeal (MCP) and radiocarpal (RC) joints. Amikacin was quantified by a fluorescence polarization immunoassay. Regardless of the group, no significant difference in the synovial amikacin concentrations was noted between 20 and 30 min RLP. Mean synovial concentrations of amikacin in the standing sedated horses were significantly higher in the MCP joint at 30 min (P = 0.003) compared to horses under general anesthesia. No significant difference was noted for the RC joint.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalVeterinary Journal
Volume208
DOIs
StatePublished - Feb 1 2016

Fingerprint

tourniquets
amikacin
Tourniquets
Amikacin
limbs (animal)
Horses
Extremities
Perfusion
General Anesthesia
horses
sedation
anesthesia
joints (animal)
Metacarpophalangeal Joint
pharmacokinetics
Pharmacokinetics
Fluorescence Polarization Immunoassay
synovial fluid
carpus
Synovial Fluid

Keywords

  • Amikacin
  • Horses
  • Infection
  • Regional limb perfusion
  • Tourniquet

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Comparison of two tourniquet application times for regional intravenous limb perfusions with amikacin in sedated or anesthetized horses. / Aristizabal, F. A.; Nieto, Jorge; Guedes, A. G.; Dechant, Julie E; Yamout, S.; Morales, B.; Snyder, J.

In: Veterinary Journal, Vol. 208, 01.02.2016, p. 50-54.

Research output: Contribution to journalArticle

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