Abstract
Objective - To determine the optimal osteogenic source of equine mesenchymal stem cells (eMSCs) and optimize collection of and expansion conditions for those cells. Animals - 10 adult Quarter Horses and 8 newborn Thoroughbred foals. Procedures - eMSCs were isolated from bone marrow (BM), adipose tissue, and umbilical cord blood and tissue, and the osteogenic potential of each type was assessed. Effects of anatomic site, aspiration volume, and serum type on eMSC yield from BM were investigated. Results - BM-eMSCs had the highest overall expression of the osteogenic genes Cbfa1, Osx, and Omd and staining for ALP activity and calcium deposition. There was no significant difference in BM-eMSC yield from the tuber coxae or sternum, but yield was significantly greater from the first 60-mL aspirate than from subsequent aspirates. The BM-eMSC expansion rate was significantly higher when cells were cultured in fetal bovine serum instead of autologous serum (AS). Conclusions and Clinical Relevance - eMSCs from BM possessed the highest in vitro osteogenic potential; eMSCs from adipose tissue also had robust osteogenic potential. The tuber coxae and the sternum were viable sources of BM-eMSCs in yearlings, and 60 mL of BM aspirate was sufficient for culture and expansion. Expanding BM-eMSCs in AS to avoid potential immunologic reactions decreased the total yield because BM-eMSCs grew significantly slower in AS than in fetal bovine serum. Additional studies are needed to determine optimal ex vivo eMSC culture and expansion conditions, including the timing and use of growth factor-supplemented AS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1237-1245 |
| Number of pages | 9 |
| Journal | American Journal of Veterinary Research |
| Volume | 71 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2010 |
Fingerprint
ASJC Scopus subject areas
- veterinary(all)
Cite this
Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissue. / Toupadakis, Chrisoula A.; Wong, Alice; Genetos, Damian C; Cheung, Whitney K.; Borjesson, Dori L; Ferraro, Gregory L.; Galuppo, Larry D; Leach, Jonathan K; Owens, Sean D; Yellowley-genetos, Clare E.
In: American Journal of Veterinary Research, Vol. 71, No. 10, 10.2010, p. 1237-1245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissue
AU - Toupadakis, Chrisoula A.
AU - Wong, Alice
AU - Genetos, Damian C
AU - Cheung, Whitney K.
AU - Borjesson, Dori L
AU - Ferraro, Gregory L.
AU - Galuppo, Larry D
AU - Leach, Jonathan K
AU - Owens, Sean D
AU - Yellowley-genetos, Clare E
PY - 2010/10
Y1 - 2010/10
N2 - Objective - To determine the optimal osteogenic source of equine mesenchymal stem cells (eMSCs) and optimize collection of and expansion conditions for those cells. Animals - 10 adult Quarter Horses and 8 newborn Thoroughbred foals. Procedures - eMSCs were isolated from bone marrow (BM), adipose tissue, and umbilical cord blood and tissue, and the osteogenic potential of each type was assessed. Effects of anatomic site, aspiration volume, and serum type on eMSC yield from BM were investigated. Results - BM-eMSCs had the highest overall expression of the osteogenic genes Cbfa1, Osx, and Omd and staining for ALP activity and calcium deposition. There was no significant difference in BM-eMSC yield from the tuber coxae or sternum, but yield was significantly greater from the first 60-mL aspirate than from subsequent aspirates. The BM-eMSC expansion rate was significantly higher when cells were cultured in fetal bovine serum instead of autologous serum (AS). Conclusions and Clinical Relevance - eMSCs from BM possessed the highest in vitro osteogenic potential; eMSCs from adipose tissue also had robust osteogenic potential. The tuber coxae and the sternum were viable sources of BM-eMSCs in yearlings, and 60 mL of BM aspirate was sufficient for culture and expansion. Expanding BM-eMSCs in AS to avoid potential immunologic reactions decreased the total yield because BM-eMSCs grew significantly slower in AS than in fetal bovine serum. Additional studies are needed to determine optimal ex vivo eMSC culture and expansion conditions, including the timing and use of growth factor-supplemented AS.
AB - Objective - To determine the optimal osteogenic source of equine mesenchymal stem cells (eMSCs) and optimize collection of and expansion conditions for those cells. Animals - 10 adult Quarter Horses and 8 newborn Thoroughbred foals. Procedures - eMSCs were isolated from bone marrow (BM), adipose tissue, and umbilical cord blood and tissue, and the osteogenic potential of each type was assessed. Effects of anatomic site, aspiration volume, and serum type on eMSC yield from BM were investigated. Results - BM-eMSCs had the highest overall expression of the osteogenic genes Cbfa1, Osx, and Omd and staining for ALP activity and calcium deposition. There was no significant difference in BM-eMSC yield from the tuber coxae or sternum, but yield was significantly greater from the first 60-mL aspirate than from subsequent aspirates. The BM-eMSC expansion rate was significantly higher when cells were cultured in fetal bovine serum instead of autologous serum (AS). Conclusions and Clinical Relevance - eMSCs from BM possessed the highest in vitro osteogenic potential; eMSCs from adipose tissue also had robust osteogenic potential. The tuber coxae and the sternum were viable sources of BM-eMSCs in yearlings, and 60 mL of BM aspirate was sufficient for culture and expansion. Expanding BM-eMSCs in AS to avoid potential immunologic reactions decreased the total yield because BM-eMSCs grew significantly slower in AS than in fetal bovine serum. Additional studies are needed to determine optimal ex vivo eMSC culture and expansion conditions, including the timing and use of growth factor-supplemented AS.
UR - http://www.scopus.com/inward/record.url?scp=77958605841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77958605841&partnerID=8YFLogxK
U2 - 10.2460/ajvr.71.10.1237
DO - 10.2460/ajvr.71.10.1237
M3 - Article
C2 - 20919913
AN - SCOPUS:77958605841
VL - 71
SP - 1237
EP - 1245
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 10
ER -