Comparison of the internal transcribed spacer, ITS-1, from Sarcocystis falcatula isolates and Sarcocystis neurona

A. E. Marsh, B. C. Barr, Lisa A Tell, D. D. Bowman, Patricia A Conrad, C. Ketcherside, T. Green

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The genetic diversity among 6 Sarcocystis falcatula isolates derived from geographically distinct regions in the U.S.A. was detected using the first internal transcribed spacer region 1 (ITS-1) of the rRNA gene. These sequences were then compared to the full sequence from a Sarcocystis neurona isolate obtained from a California horse diagnosed with equine protozoal myeloencephalitis. No nucleotide differences were detected over partial sequence analysis of 2 additional S. neurona isolates; however, the complete nucleotide sequence for the ITS-1 region was not compared. Twelve nucleotide differences were consistently detected when aligned sequences of S. neurona were compared to those of the S. falcatula isolates. Additional nucleotide base changes were detected among the S. falcatula isolates, but these changes were not consistent in all the S. falcatula isolates. These results indicate that S. falcatula may be comprised of a heterogeneous population and that the ITS-1 region can be used to distinguish S. neurona from S. falcatula used in this study.

Original languageEnglish (US)
Pages (from-to)750-757
Number of pages8
JournalJournal of Parasitology
Volume85
Issue number4
DOIs
StatePublished - Aug 1999

Fingerprint

Sarcocystis falcatula
Sarcocystis
Sarcocystis neurona
internal transcribed spacers
Nucleotides
Horses
Encephalomyelitis
nucleotides
rRNA Genes
Sequence Analysis
equine protozoal myeloencephalitis
horse
Population
comparison
sequence analysis
ribosomal RNA
horses
nucleotide sequences
genetic variation
gene

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Comparison of the internal transcribed spacer, ITS-1, from Sarcocystis falcatula isolates and Sarcocystis neurona. / Marsh, A. E.; Barr, B. C.; Tell, Lisa A; Bowman, D. D.; Conrad, Patricia A; Ketcherside, C.; Green, T.

In: Journal of Parasitology, Vol. 85, No. 4, 08.1999, p. 750-757.

Research output: Contribution to journalArticle

Marsh, A. E. ; Barr, B. C. ; Tell, Lisa A ; Bowman, D. D. ; Conrad, Patricia A ; Ketcherside, C. ; Green, T. / Comparison of the internal transcribed spacer, ITS-1, from Sarcocystis falcatula isolates and Sarcocystis neurona. In: Journal of Parasitology. 1999 ; Vol. 85, No. 4. pp. 750-757.
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