Comparison of the effect of post-heparin and pre-heparin lipoprotein lipase and hepatic triglyceride lipase on remnant lipoprotein metabolism

Takashi Shirakawa, Katsuyuki Nakajima, Younosuke Shimomura, Junji Kobayashi, Kimber Stanhope, Peter J Havel, Tetsuo Machida, Hiroyuki Sumino, Masami Murakami

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Background: A comparison of post-heparin and pre-heparin plasma lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) on the metabolism of remnant lipoproteins (RLPs) has not been reported yet. Methods: Healthy volunteers were injected with heparin for LPL and HTGL determination in the fasting (8:00) and postprandial (20:00) plasma on the same day. Plasma total cholesterol (TC), triglycerides (TG), LDL-C, HDL-C, small dense LDL (sdLDL)-C, remnant lipoprotein (RLP)-C, RLP-TG, the RLP-TG/RLP-C ratio, adiponectin and apoCIII were measured. Results: LPL activity and concentration in the post-heparin plasma exhibited a significant inverse correlation with TG, RLP-C, RLP-TG, and RLP particle size estimated as RLP-TG/RLP-C ratio and sdLDL-C, and positively correlated with HDL-C. HTGL was only inversely correlated with HDL-C. LPL concentration in the pre-heparin plasma was also inversely correlated with the RLP-TG/RLP-C ratio and other lipoprotein parameters. Adiponectin was inversely correlated with RLP-TG/RLP-C ratio and apoC III was positively correlated with RLP-TG/RLP-C ratio, but not correlated with LPL activity. Conclusion: LPL activity and concentration were inversely and significantly correlated with the particle size of RLP in both the post-heparin and pre-heparin plasma. Those results suggest that LPL concentration in pre-heparin plasma can take the place of LPL activity in the post-heparin plasma.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalClinica Chimica Acta
Volume440
DOIs
StatePublished - Feb 2 2015

Keywords

  • Hepatic triglyceride lipase (HTGL)
  • Lipoprotein lipase (LPL)
  • Post-heparin plasma
  • Pre-heparin plasma
  • Remnant lipoproteins (RLP)
  • RLP-TG/RLP-C ratio

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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