Comparison of the BioRad Variant and Primus Ultra2 high-pressure liquid chromatography (HPLC) instruments for the detection of variant hemoglobins

R. C. Gosselin, A. C. Carlin, Denis M Dwyre

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Introduction: Hemoglobin variants are a result of genetic changes resulting in abnormal or dys-synchronous hemoglobin chain production (thalassemia) or the generation of hemoglobin chain variants such as hemoglobin S. Automated high-pressure liquid chromatography (HPLC) systems have become the method of choice for the evaluation of patients suspected with hemoglobinopathies. Methods: In this study, we evaluated the performance of two HPLC methods used in the detection of common hemoglobin variants: Variant and Ultra2. Results: There were 377 samples tested, 26% (99/377) with HbS, 8.5% (32/377) with HbC, 20.7% (78/377) with other hemoglobin variant or thalassemia, and 2.9% with increased hemoglobin A1c. The interpretations of each chromatograph were compared. There were no differences noted for hemoglobins A0, S, or C. There were significant differences between HPLC methods for hemoglobins F, A2, and A1c. However, there was good concordance between normal and abnormal interpretations (97.9% and 96.2%, respectively). Conclusion: Both Variant and Ultra2 HPLC methods were able to detect most common hemoglobin variants. There was better discrimination for fast hemoglobins, between hemoglobins E and A2, and between hemoglobins S and F using the Ultra2 HPLC method.

Original languageEnglish (US)
Pages (from-to)159-167
Number of pages9
JournalInternational Journal of Laboratory Hematology
Volume33
Issue number2
DOIs
StatePublished - Apr 2011

Fingerprint

High pressure liquid chromatography
Hemoglobins
High Pressure Liquid Chromatography
Sickle Hemoglobin
Hemoglobin A2
Fetal Hemoglobin
Thalassemia
Hemoglobin E
Hemoglobin C
Hemoglobinopathies

Keywords

  • BioRad Variant
  • Hemoglobinopathy
  • High-pressure liquid chromatography
  • Primus Ultra2

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Hematology

Cite this

@article{d98d6218ea014ac6b5866e669ff29475,
title = "Comparison of the BioRad Variant and Primus Ultra2 high-pressure liquid chromatography (HPLC) instruments for the detection of variant hemoglobins",
abstract = "Introduction: Hemoglobin variants are a result of genetic changes resulting in abnormal or dys-synchronous hemoglobin chain production (thalassemia) or the generation of hemoglobin chain variants such as hemoglobin S. Automated high-pressure liquid chromatography (HPLC) systems have become the method of choice for the evaluation of patients suspected with hemoglobinopathies. Methods: In this study, we evaluated the performance of two HPLC methods used in the detection of common hemoglobin variants: Variant and Ultra2. Results: There were 377 samples tested, 26{\%} (99/377) with HbS, 8.5{\%} (32/377) with HbC, 20.7{\%} (78/377) with other hemoglobin variant or thalassemia, and 2.9{\%} with increased hemoglobin A1c. The interpretations of each chromatograph were compared. There were no differences noted for hemoglobins A0, S, or C. There were significant differences between HPLC methods for hemoglobins F, A2, and A1c. However, there was good concordance between normal and abnormal interpretations (97.9{\%} and 96.2{\%}, respectively). Conclusion: Both Variant and Ultra2 HPLC methods were able to detect most common hemoglobin variants. There was better discrimination for fast hemoglobins, between hemoglobins E and A2, and between hemoglobins S and F using the Ultra2 HPLC method.",
keywords = "BioRad Variant, Hemoglobinopathy, High-pressure liquid chromatography, Primus Ultra2",
author = "Gosselin, {R. C.} and Carlin, {A. C.} and Dwyre, {Denis M}",
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N2 - Introduction: Hemoglobin variants are a result of genetic changes resulting in abnormal or dys-synchronous hemoglobin chain production (thalassemia) or the generation of hemoglobin chain variants such as hemoglobin S. Automated high-pressure liquid chromatography (HPLC) systems have become the method of choice for the evaluation of patients suspected with hemoglobinopathies. Methods: In this study, we evaluated the performance of two HPLC methods used in the detection of common hemoglobin variants: Variant and Ultra2. Results: There were 377 samples tested, 26% (99/377) with HbS, 8.5% (32/377) with HbC, 20.7% (78/377) with other hemoglobin variant or thalassemia, and 2.9% with increased hemoglobin A1c. The interpretations of each chromatograph were compared. There were no differences noted for hemoglobins A0, S, or C. There were significant differences between HPLC methods for hemoglobins F, A2, and A1c. However, there was good concordance between normal and abnormal interpretations (97.9% and 96.2%, respectively). Conclusion: Both Variant and Ultra2 HPLC methods were able to detect most common hemoglobin variants. There was better discrimination for fast hemoglobins, between hemoglobins E and A2, and between hemoglobins S and F using the Ultra2 HPLC method.

AB - Introduction: Hemoglobin variants are a result of genetic changes resulting in abnormal or dys-synchronous hemoglobin chain production (thalassemia) or the generation of hemoglobin chain variants such as hemoglobin S. Automated high-pressure liquid chromatography (HPLC) systems have become the method of choice for the evaluation of patients suspected with hemoglobinopathies. Methods: In this study, we evaluated the performance of two HPLC methods used in the detection of common hemoglobin variants: Variant and Ultra2. Results: There were 377 samples tested, 26% (99/377) with HbS, 8.5% (32/377) with HbC, 20.7% (78/377) with other hemoglobin variant or thalassemia, and 2.9% with increased hemoglobin A1c. The interpretations of each chromatograph were compared. There were no differences noted for hemoglobins A0, S, or C. There were significant differences between HPLC methods for hemoglobins F, A2, and A1c. However, there was good concordance between normal and abnormal interpretations (97.9% and 96.2%, respectively). Conclusion: Both Variant and Ultra2 HPLC methods were able to detect most common hemoglobin variants. There was better discrimination for fast hemoglobins, between hemoglobins E and A2, and between hemoglobins S and F using the Ultra2 HPLC method.

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