Comparison of systemic oxygen delivery and uptake with NIR spectroscopy of brain during normovolemic hemodilution in the rabbit

David Lubarsky, Jack A. Griebel, Enrico M. Carnporesi, Claude A. Piantadosi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Incremental hyperoxic normovolemic hemodilution was utilized to progressively decrease oxygen delivery (Do2) in anesthetized rabbits. At decreasing D02, we compared systemic responses related to the adequacy of Do2, i.e. mixed venous oxygen saturation (SvO2), oxygen consumption (V02), and arterial lactate concentrations, to near infrared spectroscopy (NIRS) of the brain, a regional measure of intracellular oxygen availability. We sought concomitantly to define critical SvO2 and D02, beyond which whole body V02 begins to decline and arterial lactate concentrations increase. NIR Spectroscopy provided the means to test the hypothesis that systemic indicators of inadequate Doe would not accurately reflect the oxygenation of a critical organ such as the brain. In thirteen rabbits anesthetized with fentanyl, paralyzed and artificially ventilated at an FIO2 of 0.60, hemodilution produced an early decrease in mixed venous oxygen saturation. When mixed venous oxygen saturation decreased below approximately 50'%, arterial lactate concentrations began to increase significantly. Further decreases in oxygen delivery precipitated a decline in systemic Vo2. Finally, NIRS revealed an increase in the reduction level of brain cytochrome a,a3 after systemic parameters of oxygen delivery had been altered. Analysis of the data indicated that falling SvO2 predicted inadequate D02 to tissue during early hemodilution under narcotic/relaxant anesthesia and that the brain showed evidence of intracellular hypoxia only after systemic parameters such as SvO2 were affected markedly.

Original languageEnglish (US)
Pages (from-to)45-57
Number of pages13
JournalResuscitation
Volume23
Issue number1
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Hemodilution
Near-Infrared Spectroscopy
Oxygen
Rabbits
Brain
Lactic Acid
Narcotics
Fentanyl
Electron Transport Complex IV
Oxygen Consumption
Anesthesia

Keywords

  • anesthesia
  • mixed venous oxygen saturation
  • near infrared spectroscopy
  • oxygen consumption

ASJC Scopus subject areas

  • Emergency Medicine
  • Emergency
  • Cardiology and Cardiovascular Medicine

Cite this

Comparison of systemic oxygen delivery and uptake with NIR spectroscopy of brain during normovolemic hemodilution in the rabbit. / Lubarsky, David; Griebel, Jack A.; Carnporesi, Enrico M.; Piantadosi, Claude A.

In: Resuscitation, Vol. 23, No. 1, 01.01.1992, p. 45-57.

Research output: Contribution to journalArticle

Lubarsky, David ; Griebel, Jack A. ; Carnporesi, Enrico M. ; Piantadosi, Claude A. / Comparison of systemic oxygen delivery and uptake with NIR spectroscopy of brain during normovolemic hemodilution in the rabbit. In: Resuscitation. 1992 ; Vol. 23, No. 1. pp. 45-57.
@article{fbccce9fb8474135ac05204c8916fd97,
title = "Comparison of systemic oxygen delivery and uptake with NIR spectroscopy of brain during normovolemic hemodilution in the rabbit",
abstract = "Incremental hyperoxic normovolemic hemodilution was utilized to progressively decrease oxygen delivery (Do2) in anesthetized rabbits. At decreasing D02, we compared systemic responses related to the adequacy of Do2, i.e. mixed venous oxygen saturation (SvO2), oxygen consumption (V02), and arterial lactate concentrations, to near infrared spectroscopy (NIRS) of the brain, a regional measure of intracellular oxygen availability. We sought concomitantly to define critical SvO2 and D02, beyond which whole body V02 begins to decline and arterial lactate concentrations increase. NIR Spectroscopy provided the means to test the hypothesis that systemic indicators of inadequate Doe would not accurately reflect the oxygenation of a critical organ such as the brain. In thirteen rabbits anesthetized with fentanyl, paralyzed and artificially ventilated at an FIO2 of 0.60, hemodilution produced an early decrease in mixed venous oxygen saturation. When mixed venous oxygen saturation decreased below approximately 50'{\%}, arterial lactate concentrations began to increase significantly. Further decreases in oxygen delivery precipitated a decline in systemic Vo2. Finally, NIRS revealed an increase in the reduction level of brain cytochrome a,a3 after systemic parameters of oxygen delivery had been altered. Analysis of the data indicated that falling SvO2 predicted inadequate D02 to tissue during early hemodilution under narcotic/relaxant anesthesia and that the brain showed evidence of intracellular hypoxia only after systemic parameters such as SvO2 were affected markedly.",
keywords = "anesthesia, mixed venous oxygen saturation, near infrared spectroscopy, oxygen consumption",
author = "David Lubarsky and Griebel, {Jack A.} and Carnporesi, {Enrico M.} and Piantadosi, {Claude A.}",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/0300-9572(92)90161-5",
language = "English (US)",
volume = "23",
pages = "45--57",
journal = "Resuscitation",
issn = "0300-9572",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Comparison of systemic oxygen delivery and uptake with NIR spectroscopy of brain during normovolemic hemodilution in the rabbit

AU - Lubarsky, David

AU - Griebel, Jack A.

AU - Carnporesi, Enrico M.

AU - Piantadosi, Claude A.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Incremental hyperoxic normovolemic hemodilution was utilized to progressively decrease oxygen delivery (Do2) in anesthetized rabbits. At decreasing D02, we compared systemic responses related to the adequacy of Do2, i.e. mixed venous oxygen saturation (SvO2), oxygen consumption (V02), and arterial lactate concentrations, to near infrared spectroscopy (NIRS) of the brain, a regional measure of intracellular oxygen availability. We sought concomitantly to define critical SvO2 and D02, beyond which whole body V02 begins to decline and arterial lactate concentrations increase. NIR Spectroscopy provided the means to test the hypothesis that systemic indicators of inadequate Doe would not accurately reflect the oxygenation of a critical organ such as the brain. In thirteen rabbits anesthetized with fentanyl, paralyzed and artificially ventilated at an FIO2 of 0.60, hemodilution produced an early decrease in mixed venous oxygen saturation. When mixed venous oxygen saturation decreased below approximately 50'%, arterial lactate concentrations began to increase significantly. Further decreases in oxygen delivery precipitated a decline in systemic Vo2. Finally, NIRS revealed an increase in the reduction level of brain cytochrome a,a3 after systemic parameters of oxygen delivery had been altered. Analysis of the data indicated that falling SvO2 predicted inadequate D02 to tissue during early hemodilution under narcotic/relaxant anesthesia and that the brain showed evidence of intracellular hypoxia only after systemic parameters such as SvO2 were affected markedly.

AB - Incremental hyperoxic normovolemic hemodilution was utilized to progressively decrease oxygen delivery (Do2) in anesthetized rabbits. At decreasing D02, we compared systemic responses related to the adequacy of Do2, i.e. mixed venous oxygen saturation (SvO2), oxygen consumption (V02), and arterial lactate concentrations, to near infrared spectroscopy (NIRS) of the brain, a regional measure of intracellular oxygen availability. We sought concomitantly to define critical SvO2 and D02, beyond which whole body V02 begins to decline and arterial lactate concentrations increase. NIR Spectroscopy provided the means to test the hypothesis that systemic indicators of inadequate Doe would not accurately reflect the oxygenation of a critical organ such as the brain. In thirteen rabbits anesthetized with fentanyl, paralyzed and artificially ventilated at an FIO2 of 0.60, hemodilution produced an early decrease in mixed venous oxygen saturation. When mixed venous oxygen saturation decreased below approximately 50'%, arterial lactate concentrations began to increase significantly. Further decreases in oxygen delivery precipitated a decline in systemic Vo2. Finally, NIRS revealed an increase in the reduction level of brain cytochrome a,a3 after systemic parameters of oxygen delivery had been altered. Analysis of the data indicated that falling SvO2 predicted inadequate D02 to tissue during early hemodilution under narcotic/relaxant anesthesia and that the brain showed evidence of intracellular hypoxia only after systemic parameters such as SvO2 were affected markedly.

KW - anesthesia

KW - mixed venous oxygen saturation

KW - near infrared spectroscopy

KW - oxygen consumption

UR - http://www.scopus.com/inward/record.url?scp=0026506392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026506392&partnerID=8YFLogxK

U2 - 10.1016/0300-9572(92)90161-5

DO - 10.1016/0300-9572(92)90161-5

M3 - Article

C2 - 1315069

AN - SCOPUS:0026506392

VL - 23

SP - 45

EP - 57

JO - Resuscitation

JF - Resuscitation

SN - 0300-9572

IS - 1

ER -