Comparison of six D-dimer methods in patients suspected of deep vein thrombosis

Robert C. Gosselin, John T Owings, Joshua Kehoe, John T Anderson, Denis M Dwyre, Robert C. Jacoby, Garth H Utter, Edward C. Larkin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We evaluated six D-dimer methods to determine their sensitivity, specificity, and negative predictive values (NPV) in symptomatic patients suspected of deep vein thrombosis (DVT). In patients suspected of DVT a whole blood D-dimer test (SimpliRED, Agen) was performed, and then tested using enzyme-linked immunosorbent assay (VIDAS D-Dimer, BioMerieux; Asserachrome D-Di, Stago International; Dimertest Gold, Agen) and automated immunoturbidometric methods (Advanced D-Dimer, Dade Behring; MiniQuant, Biopool). Each D-dimer method was independently compared with radiographic results to determine sensitivity and NPV. There were 151 patients enrolled in the study. Thirty-five (23.2%) patients had a positive Doppler ultrasound, with 26 proximal, eight distal, and one patient with both proximal and distal thrombus. Two patients (1.3%) had inconclusive studies and were excluded from the analyses. For all patients, the sensitivities for the rapid D-dimer methods were: SimpliRED, 82. 3% [95% confidence interval (CI), 80,3-84.3%]; VIDAS D-Dimer, 91.4% (95% CI, 89.9-92.9%); MiniQuant D-Dimer, 96.3% (95% CI, 95.1 -97,5%); and Advanced D-Dimer, 97.1% (95% CI, 96.3-97.9%). The sensitivity improved for SimpliRED (86.4%; 95% CI, 83.3-89.4%), VIDAS D-Dimer (95.5%; 95% CI, 85.0-100%), MiniQuant D-Dimer (100%; 95% CI, 96.9-100%) and Advanced D-Dimer (100%; 95% CI, 98.9-100%) in the inpatient population. The automated immunoturbidometric methods, the MiniQuant D-Dimer and Advanced D-Dimer, demonstrated comparable sensitivities and NPV with the VIDAS D-Dimer method in symptomatic patients suspected of DVT, which would suggest that these newer D-dimer methods could be used as part of the diagnostic algorithm for patients suspected of DVT.

Original languageEnglish (US)
Pages (from-to)545-550
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume14
Issue number6
DOIs
StatePublished - Sep 2003

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Keywords

  • Advanced D-Dimer
  • Deep vein thrombosis
  • MiniQuant D-Dimer
  • Pulmonary embolism
  • SimpliRED
  • VIDAS D-Dimer

ASJC Scopus subject areas

  • Hematology

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