Comparison of serum estradiol to urinary estrone conjugates in the rhesus macaque (Macaca mulatta).

S. L. Monfort, D. L. Hess, S. E. Shideler, S. J. Samuels, Andrew G Hendrickx, B. L. Lasley

Research output: Contribution to journalArticle

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Abstract

Paired urine and serum samples were collected daily during fourteen nonconceptive (7 females) and ten conceptive (9 females) ovarian cycles from a total of 12 female rhesus monkeys (Macaca mulatta). Daily urine samples were analyzed for concentrations of estrone conjugates (Ei Conj). Serum samples were evaluated for concentrations of estradiol (E2) and progesterone (P) by radioimmunoassay (RIA), and bioactive luteinizing hormone (bLH) and monkey chorionic gonadotropin (mCG) were analyzed by a mouse Leydig cell bioassay. Linear correlation (r) between urinary E1 Conj and serum E2 (r range = -0.176-0.948) during nonconceptive cycles aligned by the preovulatory E1 Conj peak (Day 0) improved when daily hormone values were realigned to account for an approximately 24-h delay in the excretion of hormonal metabolites in urine (r range = 0.465-0.967). Similarly, correlation between urinary E1 Conj and serum E2 during conceptive cycles aligned by Day 0 (r range = 0.300-0.824) improved when values were offset by 24 h (r range = 0.408-0.876). When conceptive cycles were compared to nonconceptive cycles, serum P levels were significantly elevated over nonconceptive levels by Day +12 (p less than 0.001), and urinary E1 Conj levels by Day +13 (p less than 0.02), whereas serum E2 and bLH were both significantly elevated by Day +14 (p less than 0.0006 and p less than 0.01, respectively). In both nonconceptive and conceptive cycles, urinary E1 Conj paralleled serum E2 and demonstrated incremental increases above baseline levels, which were greater than for serum E2.

Original languageEnglish (US)
Pages (from-to)832-837
Number of pages6
JournalBiology of Reproduction
Volume37
Issue number4
StatePublished - Nov 1987
Externally publishedYes

Fingerprint

Estrone
Macaca mulatta
Estradiol
Serum
Urine
Luteinizing Hormone
Leydig Cells
Chorionic Gonadotropin
Menstrual Cycle
Biological Assay
Radioimmunoassay
Haplorhini
Progesterone
Hormones

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Monfort, S. L., Hess, D. L., Shideler, S. E., Samuels, S. J., Hendrickx, A. G., & Lasley, B. L. (1987). Comparison of serum estradiol to urinary estrone conjugates in the rhesus macaque (Macaca mulatta). Biology of Reproduction, 37(4), 832-837.

Comparison of serum estradiol to urinary estrone conjugates in the rhesus macaque (Macaca mulatta). / Monfort, S. L.; Hess, D. L.; Shideler, S. E.; Samuels, S. J.; Hendrickx, Andrew G; Lasley, B. L.

In: Biology of Reproduction, Vol. 37, No. 4, 11.1987, p. 832-837.

Research output: Contribution to journalArticle

Monfort, SL, Hess, DL, Shideler, SE, Samuels, SJ, Hendrickx, AG & Lasley, BL 1987, 'Comparison of serum estradiol to urinary estrone conjugates in the rhesus macaque (Macaca mulatta).', Biology of Reproduction, vol. 37, no. 4, pp. 832-837.
Monfort SL, Hess DL, Shideler SE, Samuels SJ, Hendrickx AG, Lasley BL. Comparison of serum estradiol to urinary estrone conjugates in the rhesus macaque (Macaca mulatta). Biology of Reproduction. 1987 Nov;37(4):832-837.
Monfort, S. L. ; Hess, D. L. ; Shideler, S. E. ; Samuels, S. J. ; Hendrickx, Andrew G ; Lasley, B. L. / Comparison of serum estradiol to urinary estrone conjugates in the rhesus macaque (Macaca mulatta). In: Biology of Reproduction. 1987 ; Vol. 37, No. 4. pp. 832-837.
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