Comparison of sensitivity of immunofluorescent microscopy to that of a combination of immunofluorescent microscopy and immunomagnetic separation for detection of Cryptosporidium parvum oocysts in adult bovine feces

Maria Das Graças C Pereira, Edward R Atwill, Ted Jones

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A direct immunofluorescence assay (DFA) (Merifluor; Meridian Diagnostics, Inc., Cincinnati, Ohio) was compared to an immunomagnetic separation (IMS) assay (Dynabeads; Dynal, Inc., Lake Success, N.Y.) coupled with immunofluorescent microscopy (Waterborne, Inc., New Orleans, La.) for their ability to detect low concentrations of Cryptosporidium parvum oocysts in adult bovine fecal material. IMS-DFA resulted in a 2-log-unit increase in sensitivity (10 oocysts/g) compared to DFA alone (1,000 oocysts/g). The higher sensitivity obtained with IMS-DFA resulted from testing 2 g of fecal material instead of the 13 to 19 mg of fecal material tested in the DFA; the increased sensitivity was not attributable to a higher percent recovery.

Original languageEnglish (US)
Pages (from-to)3236-3239
Number of pages4
JournalApplied and Environmental Microbiology
Volume65
Issue number7
StatePublished - Jul 1999

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

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